Construct: ORF TRCN0000474792
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003379.1_s317c1
- Derived from:
- ccsbBroadEn_07387
- DNA Barcode:
- AGAGGGCTCGGCTTGAACCCCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR52 (9293)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474792
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9293 | GPR52 | G protein-coupled receptor 52 | NM_005684.5 | 99.9% | 100% | 693T>C |
| 2 | mouse | 620246 | Gpr52 | G protein-coupled receptor 52 | NM_001146330.1 | 91.5% | 95.5% | (many diffs) |
| 3 | mouse | 620246 | Gpr52 | G protein-coupled receptor 52 | XM_017321886.1 | 91.5% | 95.5% | (many diffs) |
| 4 | mouse | 620246 | Gpr52 | G protein-coupled receptor 52 | XM_017321888.1 | 91.5% | 95.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1149
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgaatccagg tggactgaat ggaggatcct gaacatgagc agtggcattg 121 tgaatgtgtc cgagcgtcac tcctgcccac ttggatttgg ccactacagt gtggtggatg 181 tctgcatctt cgagacagtg gttattgtgt tgctgacatt tctgatcatt gctgggaatc 241 taacagttat ctttgtcttt cattgtgctc cactgttaca tcattatact accagctatt 301 tcattcagac gatggcatat gctgatcttt tcgttggagt tagctgcttg gttcctactc 361 tgtcacttct ccactactcc acaggtgtcc acgagtcatt gacttgccag gtttttggat 421 atatcatctc agttctaaaa agtgtttcta tggcatgtct tgcttgcatc agtgtggatc 481 gttatcttgc aataaccaag cctctttcct acaatcaact ggtcacccct tgtcgcttga 541 gaatttgcat tattttgatc tggatctact cctgcctaat tttcttgcct tccttttttg 601 gctgggggaa acctggttac catggtgaca tttttgaatg gtgtgccacg tcttggctca 661 ccagtgccta ttttactggc tttattgttt gtttacttta tgctcctgct gcctttgttg 721 tctgcttcac ttacttccac attttcaaaa tttGCCGCCA GCACACCAAA GAGATAAATG 781 ACCGAAGAGC CCGATTCCCT AGTCATGAGG TAGATTCTTC CAGAGAGACT GGACACAGCC 841 CTGACCGTCG CTACGCCATG GTTTTGTTTA GGATAACCAG TGTATTTTAT ATGCTGTGGC 901 TCCCCTATAT AATTTACTTT CTTCTAGAAA GCTCCCGGGT CTTGGACAAT CCAACTCTGT 961 CCTTCTTAAC AACCTGGCTT GCAATAAGTA ATAGTTTTTG TAACTGTGTA ATATACAGCC 1021 TCTCCAACAG CGTTTTCCGG CTAGGCCTCC GAAGACTGTC TGAGACAATG TGCACATCCT 1081 GTATGTGTGT GAAGGATCAG GAAGCACAAG AACCCAAACC TAGGAAACGG GCTAATTCTT 1141 GCTCCATTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGAGGG CTCGGCTTGA ACCCCTGCAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt