Construct: ORF TRCN0000474798
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009217.1_s317c1
- Derived from:
- ccsbBroadEn_08051
- DNA Barcode:
- AGTAAACGAACTCCATTACTATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LAMP3 (27074)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474798
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27074 | LAMP3 | lysosomal associated membra... | NM_014398.4 | 99.7% | 99.5% | 95A>G;267G>A;952A>G |
| 2 | human | 27074 | LAMP3 | lysosomal associated membra... | XM_005247360.5 | 92.4% | 91.8% | (many diffs) |
| 3 | human | 27074 | LAMP3 | lysosomal associated membra... | XM_006713586.3 | 86.8% | 86.1% | (many diffs) |
| 4 | human | 27074 | LAMP3 | lysosomal associated membra... | XM_024453453.1 | 86.8% | 86.1% | (many diffs) |
| 5 | human | 27074 | LAMP3 | lysosomal associated membra... | XM_011512688.2 | 82.2% | 75% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1314
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ccggcagctc agcgcggcgg ccgcgctctt cgcgtccctg gccgtaattt 121 tgcacgatgg cagtcaaatg agagcaaaag catttccagg aaccagagat tattctcaac 181 ctactgcagc agcaacagta caggacataa aaaaacctgt ccagcaacca gctaagcaag 241 cacctcacca aactttagca gcaagattca tggatggtca tatcaccttt caaacagcgg 301 ccacagtaaa aattccaaca actaccccag caactacaaa aaacactgca accaccagcc 361 caattaccta caccctggtc acaacccagg ccacacccaa caactcacac acagctcctc 421 cagttactga agttacagtc ggccctagct tagcccctta ttcactgcca cccaccatca 481 ccccaccagc tcatacaact ggaaccagtt catcaaccgt cagccacaca actgggaaca 541 ccactcaacc cagtaaccag accacccttc cagcaacttt atcgatagca ctgcacaaaa 601 gcacaaccgg tcagaagcct gttcaaccca cccatgcccc aggaacaacg gcagctgccc 661 acaataccac ccgcacagct gcacctgcct ccacggttcc tgggcccacc cttgcacctc 721 agccatcgtc agtcaagact ggaatttatc aggttctaaa cggaagcaga ctctgtataa 781 aagcagagat ggggatacag ctgattgttc aagacaagga gtcggttttt tcacctcgga 841 gatacttcaa catcgacccc aacgcaacgc aagcctctgg gaactgtggc acccgaaaat 901 ccaaccttct gttgaatttt cagggcggat ttgtgaaTCT CACATTTACC AAGGATGAAG 961 AATCATATTA TATCAGTGAA GTGGGAGCCT ATTTGACCGT CTCAGATCCA GAGACAGTTT 1021 ACCAAGGAAT CAAACATGCG GTGGTGATGT TCCAGACAGC AGTCGGGCAT TCCTTCAAGT 1081 GCGTGAGTGA ACAGAGCCTC CAGTTGTCAG CCCACCTGCA GGTGAAAACA ACCGATGTCC 1141 AACTTCAAGC CTTTGATTTT GAAGATGACC ACTTTGGAAA TGTGGATGAG TGCTCGTCTG 1201 ACTACACAAT TGTGCTTCCT GTGATTGGGG CCATCGTGGT TGGTCTCTGC CTTATGGGTA 1261 TGGGTGTCTA TAAAATCCGC CTAAGGTGTC AATCATCTGG ATACCAGAGA ATCTACCCAA 1321 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1381 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1441 TATCTTGTGG AAAGGACGAA GTAAACGAAC TCCATTACTA TTGACGCGTT AAGTCgacaa 1501 tcaacctctg gattacaaaa tttgtgaaag att