Construct: ORF TRCN0000474800
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013264.1_s317c1
- Derived from:
- ccsbBroadEn_02221
- DNA Barcode:
- AGGAAACTTGTAGACTTAGAAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HS2ST1 (9653)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474800
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9653 | HS2ST1 | heparan sulfate 2-O-sulfotr... | NM_001134492.2 | 100% | 100% | |
| 2 | human | 9653 | HS2ST1 | heparan sulfate 2-O-sulfotr... | NM_012262.4 | 64.3% | 64.3% | 688_1068del |
| 3 | mouse | 23908 | Hs2st1 | heparan sulfate 2-O-sulfotr... | NM_011828.3 | 58.5% | 62.3% | (many diffs) |
| 4 | mouse | 23908 | Hs2st1 | heparan sulfate 2-O-sulfotr... | XM_006501449.2 | 58.3% | 62.1% | (many diffs) |
| 5 | mouse | 23908 | Hs2st1 | heparan sulfate 2-O-sulfotr... | XM_006501450.3 | 42.8% | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 753
- ORF length:
- 687
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gctcctcagg attatgatgc cgcccaagtt gcagctgctg gcggtggtgg 121 ccttcgcggt ggcgatgctc ttcttggaaa accagatcca gaaactggag gagtcccgct 181 cgaagctaga aagggctatt gcaagacacg aagtccgaga aattgagcag cgacatacaa 241 tggatggccc tcGGCAAGAT GCCACTTTAG ATGAGGAAGA GGACATGGTG ATCATTTATA 301 ACAGAGTTCC CAAAACGGCA AGCACTTCAT TTACCAATAT CGCCTATGAC CTGTGTGCAA 361 AGAATAAATA CCATGTCCTT CATATCAACA CTACCAAAAA TAATCCAGTG ATGTCATTGC 421 AAGATCAGGT GCGCTTTGTA AAGAATATAA CTTCCTGGAA AGAGATGAAA CCAGGATTTT 481 ATCATGGACA CGTTTCTTAC TTGGATTTTG CAAAATTTGG TGTGAAGAAG AAACCAATTT 541 ACATTAATGT CATAAGGGAT CCTATTGAGA GGCTAGTTTC TTATTATTAC TTTCTGAGAT 601 TTGGAGATGA TTATAGACCA GGGTTACGGA GACGAAAACA AGGAGACAAA AAGACCTTTG 661 ATGAATGTGT AGCAGAAGGT GGCTCAGACT GTGCTCCAGA GAAGCTCTGG CTTCAAATCC 721 CGTTCTTCTG TGGCCATAGC TCCGAATGCT GGTACCCAAC TTTCTTGTAC AAAGTGGTTG 781 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 841 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 901 GAAACTTGTA GACTTAGAAA CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 961 ttgtgaaaga tt