Construct: ORF TRCN0000474806
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005931.1_s317c1
- Derived from:
- ccsbBroadEn_00916
- DNA Barcode:
- TTCCAAGGCCCCAATCTTACTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT2 (3849)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474806
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3849 | KRT2 | keratin 2 | NM_000423.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1986
- ORF length:
- 1917
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagttgtcag atctcttgca aatctcgagg aagaggagga ggtggaggag 121 gattccgggg cttcagcagc ggctcagctg tggtgtctgg tggaagccgg agatcaactt 181 ccagcttctc ctgcttgagc cgccatggtg gtggtggcgg gggcttcggt ggaggcggct 241 ttggcagtcg gagtcttgtt ggccttggag ggaccaagag catctccatt agtgtggctg 301 gaggaggtgg tggctttggc gccgctggtg gatttggtgg cagaggaggt ggttttggag 361 gcggcagcag ctttggaggt ggcagcggct tcagtggtgg tggtttcggt ggaggcggct 421 ttggtggagg ccgctttgga ggttttgggg gccctggtgg tgttggaggt ttagggggtc 481 ctggtggctt tgggcctgga ggataccctg gtggcatcca cgaagtctct gtcaaccaga 541 gcctcctgca gcctctcaac gtgaaagttg acccagagat ccagaatgtg aaggcccaag 601 agcgtgagca gatcaaaact ctcaacaaca aatttgcctc cttcattgac aaggtgcggt 661 tcttggagca gcagaaccag gtgttacaga ccaaatggga gctgctacaa caaatgaatg 721 ttggcacccg ccccatcaac ctggagccca tcttccaggg gtatatcgac agcctcaaga 781 gatatctgga tgggctcact gcagaaagaa catcacagaa ttcagagctg aataacatgc 841 aggatcttgt ggaggattat aagaagaagt atgaggatga aatcaataag cgcacagctg 901 ctgagaatga ttttgtgacg cttaaaaagg acgtggacaa tgcctacatg ataaaggtgg 961 agttgcagtc caaggtggac ctgctgaacc aggaaattga gtttctgaaa gttctctatg 1021 atgcggagat atcccagata catcagagtg tcactgacac caacgtcatc ctctccatgg 1081 acaacagccg caacctggac ttggatagca tcatcgccga ggtcaaggcc cagtatgagg 1141 agatcgccca gaggagcaag gaagaagcgg aggccctgta ccacagcaag tatgaggagc 1201 tccaggtgac tgtcgggaga catggagaca gcctgaaaga gatcaagata gagatcagcg 1261 agctgaaccg cgtgatccag aggctgcagg gggagatcgc acatgtgaag aagcagtgta 1321 agaatgtgca agatgccatc gcagatgccg agcagcgtgg ggagcatgcc ctcaaggatg 1381 ccaggaacaa gttgaatgac ctggaggagg ccctgcagca ggccaaggag gacttggcgc 1441 ggctgctgcg tgactaccag gagctgatga acgtgaagct ggccctagat gtggagatcg 1501 ccacctaccg caaactgctg gagggcgagg agtgcaggat gtctggagac ctcagcagca 1561 atgtgactgt gtctgtgaca agcagcacca tttcatcaaa tgtggcatcc aaggctgcct 1621 ttGGAGGTTC TGGAGGTAGA GGGTCCAGTT CCGGAGGAGG ATACAGCTCT GGAAGCAGCA 1681 GTTATGGCTC TGGAGGCCGA CAGTCTGGCT CCAGAGGCGG TAGTGGAGGA GGAGGTTCTA 1741 TCTCTGGAGG AGGATATGGC TCTGGCGGTG GTTCTGGAGG AAGATACGGA TCTGGTGGTG 1801 GCTCTAAGGG AGGGTCCATC TCTGGAGGAG GATATGGCTC TGGAGGTGGA AAACACAGCT 1861 CTGGAGGTGG CTCTAGAGGA GGCTCCAGCT CTGGAGGAGG ATATGGCTCT GGAGGTGGGG 1921 GTTCTAGCTC TGTAAAGGGT AGCTCAGGTG AAGCTTTTGG TTCCAGCGTG ACCTTCTCTT 1981 TTAGATTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2041 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2101 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTCCAAGGC CCCAATCTTA CTTAAACGCG 2161 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt