Construct: ORF TRCN0000474809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006472.1_s317c1
- Derived from:
- ccsbBroadEn_13950
- DNA Barcode:
- TCTCCGCCTAGACGTCAGAGTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SELL (6402)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6402 | SELL | selectin L | NM_000655.5 | 93.8% | 93.7% | (many diffs) |
| 2 | human | 6402 | SELL | selectin L | NR_029467.2 | 45.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1191
- ORF length:
- 1125
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgcagaaaa actagagaag gaccaagcaa agccatgata tttccatgga 121 aatgtcagag cacccagagg gacttatgga acatcttcaa gttgtggggg tggacaatgc 181 tctgttgtga tttcctggca catcatggaa ccgactgctg gacttaccat tattctgaaa 241 aacccatgaa ctggcaaagg gctagaagat tctgccgaga caattacaca gatttagttg 301 ccatacaaaa caaggcggaa attgagtatc tggagaagac tctgcctttc agtcgttctt 361 actactggat aggaatccgg aagataggag gaatatggac gtgggtggga accaacaaat 421 ctcttactga agaagcagag aactggggag atggtgagcc caacaacaag aagaacaagg 481 aggactgcgt ggagatctat atcaagagaa acaaagatgc aggcaaatgg aacgatgacg 541 cctgccacaa actaaaggca gccctctgtt acacagcttc ttgccagccc tggtcatgca 601 gtggccatgg agaatgtgta gaaatcatca ataattacac cTGCAACTGT GATGTGGGGT 661 ACTATGGGCC CCAGTGTCAG TTTGTGATTC AGTGTGAGCC TTTGGAGGCC CCAGAGCTGG 721 GTACCATGGA CTGTACTCAC CCTTTGGGAA ACTTCAGCTT CAGCTCACAG TGTGCCTTCA 781 GCTGCTCTGA AGGAACAAAC TTAACTGGGA TTGAAGAAAC CACCTGTGGA CCATTTGGAA 841 ACTGGTCATC TCCAGAACCA ACCTGTCAAG TGATTCAGTG TGAGCCTCTA TCAGCACCAG 901 ATTTGGGGAT CATGAACTGT AGCCATCCCC TGGCCAGCTT CAGCTTTACC TCTGCATGTA 961 CCTTCATCTG CTCAGAAGGA ACTGAGTTAA TTGGGAAGAA GAAAACCATT TGTGAATCAT 1021 CTGGAATCTG GTCAAATCCT AGTCCAATAT GTCAAAAATT GGACAAAAGT TTCTCAATGA 1081 TTAAGGAGGG TGATTATAAC CCCCTCTTCA TTCCAGTGGC AGTCATGGTT ACTGCATTCT 1141 CTGGGTTGGC ATTTATCATT TGGCTGGCAA GGAGATTAAA AAAAGGTATG TGCCCAACTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGATCTC CGCCTAGACG TCAGAGTACG ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt