Construct: ORF TRCN0000474816
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017307.1_s317c1
- Derived from:
- ccsbBroadEn_04761
- DNA Barcode:
- TACGTCAAACCCCTGGACCTTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFI27L1 (122509)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474816
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122509 | IFI27L1 | interferon alpha inducible ... | NM_145249.3 | 100% | 100% | |
| 2 | human | 122509 | IFI27L1 | interferon alpha inducible ... | NM_206949.3 | 100% | 100% | |
| 3 | human | 122509 | IFI27L1 | interferon alpha inducible ... | XM_011536405.3 | 100% | 100% | |
| 4 | human | 122509 | IFI27L1 | interferon alpha inducible ... | XM_011536406.2 | 95.8% | 87.1% | (many diffs) |
| 5 | human | 122509 | IFI27L1 | interferon alpha inducible ... | XM_011536407.2 | 95.8% | 87.1% | (many diffs) |
| 6 | human | 122509 | IFI27L1 | interferon alpha inducible ... | XM_017020961.1 | 83.8% | 83.8% | 1_60del |
| 7 | human | 122509 | IFI27L1 | interferon alpha inducible ... | XM_017020962.2 | 75% | 75% | 1_60del;86_87ins33 |
| 8 | human | 122509 | IFI27L1 | interferon alpha inducible ... | XM_005267314.5 | 74.6% | 75.1% | (many diffs) |
| 9 | human | 122509 | IFI27L1 | interferon alpha inducible ... | XM_017020963.2 | 65.8% | 62.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 378
- ORF length:
- 312
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGGG AAAGGAGAGT GGATGGGACT CAGGCAGGGC TGCTGTAGCA GCTGTGGTCG 121 GAGGAGTTGT GGCTGTGGGG ACTGTGCTCG TGGCGCTCAG TGCCATGGGC TTCACCTCAG 181 TAGGAATCGC CGCATCCTCC ATAGCAGCCA AGATGATGTC TACAGCAGCC ATTGCCAACG 241 GGGGCGGAGT TGCTGCTGGC AGTCTGGTGG CTATTCTGCA GTCAGTGGGG GCAGCTGGAC 301 TCTCTGTGAC ATCTAAAGTT ATCGGGGGCT TTGCTGGGAC AGCTCTTGGG GCCTGGCTGG 361 GTTCACCCCC TTCCAGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 421 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 481 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATACGTCA AACCCCTGGA 541 CCTTCGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt