Construct: ORF TRCN0000474830
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015114.1_s317c1
- Derived from:
- ccsbBroadEn_14213
- DNA Barcode:
- GTTGGTTTATGTTGACGAATCGTC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KMT2E (55904)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474830
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55904 | KMT2E | lysine methyltransferase 2E | XR_001744836.2 | 40.7% | (many diffs) | |
| 2 | human | 55904 | KMT2E | lysine methyltransferase 2E | NM_018682.3 | 32.1% | 31.1% | (many diffs) |
| 3 | human | 55904 | KMT2E | lysine methyltransferase 2E | NM_182931.3 | 32.1% | 31.1% | (many diffs) |
| 4 | human | 55904 | KMT2E | lysine methyltransferase 2E | XM_005250493.1 | 32.1% | 31.1% | (many diffs) |
| 5 | human | 55904 | KMT2E | lysine methyltransferase 2E | XM_011516400.2 | 32.1% | 31.1% | (many diffs) |
| 6 | human | 55904 | KMT2E | lysine methyltransferase 2E | XM_024446837.1 | 32.1% | 31.1% | (many diffs) |
| 7 | human | 55904 | KMT2E | lysine methyltransferase 2E | XM_017012435.2 | 24.2% | 23.3% | (many diffs) |
| 8 | mouse | 69188 | Kmt2e | lysine (K)-specific methylt... | XR_389752.3 | 39.3% | (many diffs) | |
| 9 | mouse | 69188 | Kmt2e | lysine (K)-specific methylt... | XR_389753.3 | 39.3% | (many diffs) | |
| 10 | mouse | 69188 | Kmt2e | lysine (K)-specific methylt... | XM_017321090.1 | 31.1% | 31% | (many diffs) |
| 11 | mouse | 69188 | Kmt2e | lysine (K)-specific methylt... | XM_006535807.2 | 30.4% | 30.2% | (many diffs) |
| 12 | mouse | 69188 | Kmt2e | lysine (K)-specific methylt... | XM_006535806.2 | 29.7% | 29.5% | (many diffs) |
| 13 | mouse | 69188 | Kmt2e | lysine (K)-specific methylt... | NM_026984.1 | 29% | 28.9% | (many diffs) |
| 14 | mouse | 69188 | Kmt2e | lysine (K)-specific methylt... | XM_006535805.3 | 29% | 28.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1893
- ORF length:
- 1827
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag catagtgatc ccattggggg ttgatacagc agagacgtca tacttggaaa 121 tggctgcagg ttcagaacca gaatccgtag aagctagccc tgtggtagtt gagaaatcca 181 acagttatcc ccaccagtta tataccagca gctcacatca ttcacacagt tacattggtt 241 tgccctatgc ggaccataat tatggtgctc gtcctcctcc gacacctccg gcttcccctc 301 ctccatcagt ccttattagc aaaaatgaag taggcatatt taccactcct aattttgatg 361 aaacttccag tgctactaca atcagcacat ctgaggatgg aagttatggt actgatgtaa 421 ccaggtgcat atgtggtttt acacatgatg atggatacat gatctgttgt gacaaatgca 481 gcgtttggca acatattgac tgcatgggga ttgataggca gcatattcct gatacatatc 541 tatgtgaacg ttgtcagcct aggaatttgg ataaagagag ggcagtgcta ctacaacgcc 601 ggaaaaggga aaatatgtca gatggtgata ccagtgcaac tgagagtggt gatgaggttc 661 ctgtggaatt atatactgca tttcagcata ctccaacatc aattacttta actgcttcaa 721 gagtttccaa agttaatgat aaaagaagga aaaaaagcgg ggagaaagaa caacacattt 781 caaaatgtaa aaaggcattt cgtgaaggat ctaggaagtc atcaagagtt aagggttcag 841 ctccagagat tgatccttca tctgatggtt caaattttgg atgggagaca aagatcaaag 901 catggatgga tcgatatgaa gaagcaaata acaaccagta cagtgagggt gttcagaggg 961 aggcacaaag aatagctctg agattaggca atggaaatga caaaaaagag atgaataaat 1021 ccgatttgaa taccaacaat ttgctcttca aacctcctgt agagagccat atacaaaaga 1081 ataagaaaat tcttaaatct gcaaaagatt tgcctcctga tgcacttatc attgaataca 1141 gagggaagtt tatgctgaga gaacagtttg aagcaaatgg gtatttcttt aaaagaccat 1201 acccttttgt gttattctac tctaaatttc atgggctaga aatgtgtgtt gatgcaagga 1261 cttttgggaa tgaggctcga ttcatcaggc ggtcttgtac acccaatgca gaggtgaggc 1321 atgaaattca agatggaacc atacatcttt atatttattc tatacacagt attccaaagg 1381 gaactgaaat tactattgcc tttgattttg actatggaaa ttgtaagtac aaggtggact 1441 gtgcatgcct caaagaaaac ccagagtgCC CTGTTCTAAA ACGTAGTTCT GAATCCATGG 1501 AAAATATCAA TAGTGGTTAT GAGACCAGAC GGAAAAAAGG AAAAAAAGAC AAAGATATTT 1561 CAAAAGAAAA AGATACACAA AATCAGAATA TTACTTTGGA TTGTGAAGGA ACGACCAACA 1621 AAATGAAGAG CCCAGAAACT AAACAAAGAA AGCTTTCTCC ACTGAGACTA TCAGTATCAA 1681 ATAATCAGGA ACCAGATTTT ATTGATGATA TAGAAGAAAA AACTCCTATT AGTAATGAAG 1741 TAGAAATGGA ATCAGAGGAG CAGATTGCAG AAAGGAAAAG GAAGATGGTA AGTTGGGAAG 1801 CCAGTAGTTT GGGCTTAGTG ACAGCTGCTC TGCATATGGT AATTGTTGCT GCCTTTACAT 1861 GGGCTTTTAC ATTATTCTTT GAAGTTACGA ATGCCCAACT TTCTTGTACA AAGTGGTTGA 1921 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 1981 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTT 2041 GGTTTATGTT GACGAATCGT CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 2101 tgtgaaagat t