Construct: ORF TRCN0000474849
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007565.1_s317c1
- Derived from:
- ccsbBroadEn_10559
- DNA Barcode:
- CCAATAGCCAGCCCCACTTTGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOL4L-DT (149950)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474849
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 149950 | NOL4L-DT | NOL4L divergent transcript | NR_034152.1 | 48.3% | 1_232del;622A>G;653_867del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 489
- ORF length:
- 420
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggattgtaaa tccccaaagc gtgcaaacat ctgcccccat ctccctggag 121 gtggcctgtt ctccacgcca ccctcacagg ctgcctggcg gactctcctc acagcacTGT 181 GCTTCCCGGG GCCCACTTGT ACAGGCCCCA TGCGAGAAGG ACCTCGGGCA GTGTATAATC 241 CTCCAAGGGC CCACAGGAAC AGCAGTGACA ACTGTGTTAT GAAGCACCTA CTATGTGCTG 301 GGGACAAGAA TGGAACAAGA AGACATGCTT TGCCCTCACC CCTGGAGGGC AGCTTCCAGC 361 CAGGGAGGCA AATACCTCCT CCCCAGACTC CTTCCACAGA TCCCCAAACT CTGCCTCTCT 421 CCTTCAGATC CCTGCTCAGA TGTCACCAGC TCTGTGCGGC CTCCCTGCCA CCAAGTCTAA 481 AATTGCCATT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 541 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 601 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCAATA GCCAGCCCCA CTTTGCACAC 661 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt