Construct: ORF TRCN0000474858
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011936.1_s317c1
- Derived from:
- ccsbBroadEn_15956
- DNA Barcode:
- GACTAGGCGATGGCCGGCGGTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C12orf10 (60314)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474858
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60314 | C12orf10 | chromosome 12 open reading ... | NM_021640.4 | 58.5% | 58.2% | 1_465del;1046C>T;1058A>G |
| 2 | mouse | 60315 | Myg1 | melanocyte proliferating ge... | NM_021713.2 | 50.7% | 51.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 729
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gggcaccctc tatgacaaga tgtatgagaa ctttgtggag gaggtggatg 121 ctgtggacaa tgggatctcc cagtgggcag agggggagcc tcgatatgca ctgaccacta 181 ccctgagtgc acgagttgct cgacttaatc ctacctggaa ccaccccgac caagacactg 241 aggcagggtt caagcgtgca atggatctGG TTCAAGAGGA GTTTCTGCAG AGATTAGATT 301 TCTACCAACA CAGCTGGCTG CCAGCCCGGG CCTTGGTGGA AGAGGCCCTT GCCCAGCGAT 361 TCCAGGTGGA CCCAAGTGGA GAGATTGTGG AACTGGCGAA AGGTGCATGT CCCTGGAAGG 421 AGCATCTCTA CCACCTGGAA TCTGGGCTGT CCCCTCCAGT GGCCATCTTC TTTGTTATCT 481 ACACTGACCA GGCTGGACAG TGGCGAATAC AGTGTGTGCC CAAGGAGCCC CACTCATTCC 541 AAAGCCGGCT GCCCCTGCCA GAGCCATGGC GGGGTCTTCG GGACGAGGCC CTGGACCAGG 601 TCAGTGGGAT CCCTGGCTGC ATCTTCGTCC ATGCAAGCGG CTTCATTGGC GGTCACCGCA 661 CCCGAGAGGG TGCCTTGAGC ATGGCCCGTG CCACCTTGGC CCAGCGCTCA TACCTCCCAC 721 AAATCTCCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 781 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 841 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGACTAG GCGATGGCCG GCGGTGTAAC 901 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt