Construct: ORF TRCN0000474871
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016774.1_s317c1
- Derived from:
- ccsbBroadEn_12634
- DNA Barcode:
- CAAATATTCGCCGTTCCTAGGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSPP1 (79848)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474871
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_017013856.2 | 20.6% | 20.4% | 616_617delAA;627_3024del |
| 2 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_024447282.1 | 20% | 19.9% | 302_382del;697_698delAA;708_3105del |
| 3 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_017013855.2 | 20% | 19.8% | 1_246del;862_863delAA;873_3117del |
| 4 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_017013854.2 | 18.6% | 18.4% | (many diffs) |
| 5 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_006716477.3 | 18.4% | 18.3% | (many diffs) |
| 6 | human | 79848 | CSPP1 | centrosome and spindle pole... | NM_001363133.2 | 18.3% | 18.1% | 616_617delAA;627_3402del |
| 7 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_024447281.1 | 18.3% | 18.1% | 616_617delAA;627_3402del |
| 8 | human | 79848 | CSPP1 | centrosome and spindle pole... | NM_001363132.2 | 17.9% | 17.7% | 302_382del;697_698delAA;708_3483del |
| 9 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_017013852.2 | 17.5% | 17.4% | 1_246del;862_863delAA;873_3549del |
| 10 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_017013851.2 | 17.5% | 17.3% | 616_617delAA;627_3555del |
| 11 | human | 79848 | CSPP1 | centrosome and spindle pole... | NM_001364870.1 | 17.5% | 17.3% | 1_108del;724_725delAA;735_3564del |
| 12 | human | 79848 | CSPP1 | centrosome and spindle pole... | NM_001363131.2 | 17.3% | 17.1% | 616_617delAA;627_3597del |
| 13 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_024447279.1 | 17.3% | 17.1% | 616_617delAA;627_3597del |
| 14 | human | 79848 | CSPP1 | centrosome and spindle pole... | NM_024790.6 | 17% | 16.8% | 1_108del;724_725delAA;735_3663del |
| 15 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_011517607.2 | 16.9% | 16.8% | 302_382del;697_698delAA;708_3678del |
| 16 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_024447278.1 | 16.9% | 16.8% | 302_382del;697_698delAA;708_3678del |
| 17 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_011517603.2 | 16.8% | 16.6% | 302_406del;721_722delAA;732_3702del |
| 18 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_017013850.2 | 16.8% | 16.6% | 1_246del;862_863delAA;873_3702del |
| 19 | human | 79848 | CSPP1 | centrosome and spindle pole... | NM_001364869.1 | 16.6% | 16.5% | (many diffs) |
| 20 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_017013849.2 | 16.6% | 16.5% | 1_246del;862_863delAA;873_3744del |
| 21 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_006716474.3 | 16.6% | 16.4% | (many diffs) |
| 22 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_017013848.2 | 16.4% | 16.3% | (many diffs) |
| 23 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_011517602.2 | 16.4% | 16.2% | 1_246del;862_863delAA;873_3801del |
| 24 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_017013847.2 | 16.3% | 16.2% | (many diffs) |
| 25 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_011517601.2 | 16.2% | 16% | 1_246del;862_863delAA;873_3843del |
| 26 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_011517600.2 | 16% | 15.9% | (many diffs) |
| 27 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_005251305.4 | 15.9% | 15.8% | (many diffs) |
| 28 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_011517599.2 | 15.9% | 15.7% | (many diffs) |
| 29 | human | 79848 | CSPP1 | centrosome and spindle pole... | XM_011517598.2 | 15.8% | 15.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 690
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgataatttg gatgaattta ttgaagagca aaaagccaga ttggccgaag 121 acaaagcaga gttggaaagt gatccacctt acatggaaat gaagggaaag ttgtcagcga 181 agctttctga aaacagtaag atactgatct ctatggctaa ggaaaacata ccaccaaata 241 gtcaacagac caggggttcc ttaggaattg attatggatt aagtttacca cttggagaag 301 actatgaacg gaagaaaCAT AAATTAAAAG AAGAATTGCG GCAAGATTAC AGACGTTATC 361 TTACTCAGGA AAGGTTGAAA CTTGAACGTA ACAAAGAATA CAATCAGTTT CTCAGGGGTA 421 AGGAAGAATC CAGTGAAAAG TTCAGGCAGG TGGAAAAGAG TACTGAGCCC AAGAGTCAGA 481 GAAATAAAAA ACCTATTGGT CAAGTTAAGC CTGATCTAAC TTCACAAATA CAGACATCTT 541 GTGAAAATTC AGAGGGTCCT AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC 601 TTCTGAACCA AAGACGACTA GAGGAGGACA GATACCGACA ACTAGATGAT GAAATCGAAT 661 TAAGGAATAG AAGAATTATT AAAAGCAAAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 721 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 781 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAAAT 841 ATTCGCCGTT CCTAGGCGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 901 tgaaagatt