Construct: ORF TRCN0000474899
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007066.1_s317c1
- Derived from:
- ccsbBroadEn_10648
- DNA Barcode:
- ATACGGGGCGCGCTACCTGTAAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ZNRF2P1 (441208)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474899
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 100271874 | ZNRF2P2 | zinc and ring finger 2 pseu... | NR_027347.1 | 23.7% | (many diffs) | |
2 | human | 441208 | ZNRF2P1 | zinc and ring finger 2 pseu... | NR_003502.2 | 12.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 282
- ORF length:
- 213
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgcaccga aaccagcggc agtcgcacgg ccacctgagt cgccccttcc 121 TGCTGGAGCC AGCGAGGGGT GCCTGCAGCC GGGACACCTT CCTCTCCGCG TCTCCTCGTC 181 TCCCGCGTTC GCTCCTACCC TCTTGGCTCT CGGGGCACAG CGCGCGGCCC GGTCCGGAGC 241 AGGGCGGACA TGAGCGCCAG GCAGAGCGGC CTGGCCGCCG CTAACGGCGC ACGCGCGCGT 301 ACTCGGCTCG GATCTACCTT CCAGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT 361 AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA 421 CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATACGGGGCG 481 CGCTACCTGT AAGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa 541 gatt