Construct: ORF TRCN0000474908
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002528.1_s317c1
- Derived from:
- ccsbBroadEn_10333
- DNA Barcode:
- TTTCAGCAGGACCAGTTGAATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC197 (256369)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474908
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256369 | CCDC197 | coiled-coil domain containi... | NM_001351596.2 | 51.4% | 43.9% | 1_9delATGGCAGCC;363_366delGTTG;434_816del |
| 2 | human | 256369 | CCDC197 | coiled-coil domain containi... | XM_024449524.1 | 51.4% | 43.9% | 1_9delATGGCAGCC;363_366delGTTG;434_816del |
| 3 | human | 256369 | CCDC197 | coiled-coil domain containi... | XM_024449525.1 | 51.4% | 43.9% | 1_9delATGGCAGCC;363_366delGTTG;434_816del |
| 4 | human | 256369 | CCDC197 | coiled-coil domain containi... | NR_024184.2 | 33% | 1_413del;834_1270del | |
| 5 | human | 256369 | CCDC197 | coiled-coil domain containi... | NR_024183.2 | 22.4% | 1_413del;767_916del;984_1867del | |
| 6 | human | 256369 | CCDC197 | coiled-coil domain containi... | NR_024182.1 | 21.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 486
- ORF length:
- 420
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA CACAGGCCAG AGAGCTGACC CAAGCAATCC TGGTGACAAG GAAGGGGACC 121 TTCAAGGGCT GTGGCAGGAA CTCTACCAGC TCCAGGCTAA GCAGAAGAAG CTCAAGAGAG 181 AAGTCGAGAA GCACAAGCTT TTTGAAGACT ATCTGATTAA GGTCCTTGAG AAAATCCCCG 241 AGGGCTGCAC GGGATGGGAG GAGCCGGAGG AGGTGCTGGT GGAGGCCACG GTGAAGCACT 301 ACGGGAAGCT CTTCACAGCC AGCCAGGACA CGCAGAAGCG CCTCGAGGCC TTCTGCCAGA 361 TGATCCAGGC TGTCCACCGG AGCCTGGAGT CTCTGGAGGA GGACCACAGG GCTCTCATAG 421 CCTCAAGATC CGGCTGTGTC AGCTGCAGAA GAAGTGCTAC CGCAAGCAGG AGCAGTGGTG 481 GCAGCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 541 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 601 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTTCAGCAG GACCAGTTGA ATTTAACGCG 661 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt