Construct: ORF TRCN0000474913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009964.1_s317c1
- Derived from:
- ccsbBroadEn_07357
- DNA Barcode:
- CCCATGGGCACGACATATCCGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIGQ (9091)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474913
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9091 | PIGQ | phosphatidylinositol glycan... | NM_148920.3 | 99.9% | 100% | 439C>T |
| 2 | human | 9091 | PIGQ | phosphatidylinositol glycan... | NM_004204.4 | 71.7% | 68.5% | 439C>T;1531_1592del;1743_1744ins599 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2346
- ORF length:
- 2280
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gctcaaggcc ttcttcccca cgtgctgcgt ctcgacggac agcgggctgc 121 tggtgggacg gtgggtgccg gagcagagca gcgccgtggt cctggcggtc ctgcactttc 181 ccttcatccc catccaggtc aagcagctcc tggcccaggt gcggcaggcc agccaggtgg 241 gcgtggccgt gctgggcacc tggtgccact gccggcagga gcccgaggag agcctgggcc 301 gcttcctgga gagcctgggt gctgtcttcc cccatgagcc ctggctgcgg ctgtgccggg 361 agagaggcgg cacgttctgg agctgcgagg ccacccaccg gcaagcgccc actgcccccg 421 gtgcccctgg tgaggaccag gtcatgctca tcttctatga ccagcgccag gtgttgctgt 481 cacagctaca cctgcccacc gtcttgcccg accgccaggc tggagccacc actgccagca 541 cggggggcct ggctgccgtc ttcgacacgg tagcacgcag tgaggtgctc ttccgcagtg 601 accgctttga tgagggcccc gtgcggctga gccactggca gtcggagggc gtggaggcca 661 gcatcctcgc ggagctggcc aggcgagcct cgggacccat ttgcctgctg ttggccagcc 721 tgctgtcgct ggtctcagct gtcagtgcct gccgagtgtt caagctctgg cccctgtcct 781 tcctcgggag caaactctcc acgtgcgaac agctccggca ccggctggag cacctcacgc 841 taatcttcag tacacggaag gcggagaacc ctgcccagct gatgaggaag gccaacacgg 901 tggcctctgt gctgctggac gtggccctgg gcctcatgct gctgtcctgg ctccacggga 961 gaagccgcat cgggcatctg gccgacgccc tcgttcctgt ggctgaccac gtggccgagg 1021 agctccagca tctgctgcag tggctgatgg gtgctcccgc cgggctcaag atgaaccgtg 1081 cactggacca ggtgctgggc cgcttcttcc tctaccacat ccacctgtgg atcagctaca 1141 tccacctcat gtcccccttc gtggagcaca tcctttggca cgtgggcctc tcggcctgcc 1201 tgggcctgac ggtggccctg tccctcctct cggacattat cgccctcctc accttccaca 1261 tctactgctt ttacgtctat ggagccaggc tgtactgcct gaagatccat ggcctgtcct 1321 cactgtggcg tctgttccgg gggaagaagt ggaacgttct gcgccagcgc gtggactcct 1381 gttcctatga cctggaccag ctgttcatcg ggactctgct cttcaccatc ctgctcttcc 1441 tcctgcctac cacagccctg tactacctgg tgttcaccct gctccggctc ctggtggtcg 1501 ccgtgcaggg cctgatccat ctgctcgtgg acctcatcaa ctccctgccg ctgtactcac 1561 tgggtcttcg gctctgccgg ccctacaggc tggcggataa acccactgcc ctacagccgc 1621 gtggtgcaca cctaccgcct ccccagctgt ggctgccacc ccaagcactc ctggggcgcc 1681 ctgtgccgca agctgttcct tggggagctc atctacccct ggaggcagag aggggacaag 1741 caggactgag ggaactgctg gctcgcctgg caccaccaca cggccacagc cagccatctg 1801 ctctgccagg gtggcaccag ctcagctggc gcatgtcctg tgctttgtgg acgctgctgt 1861 gtgctcctga acacggcagg ccctgctatc acaccttggg cttggaggtc attgggagtg 1921 agcagatgtg ggggtggCCA GCCAGGCTGG CCGCACTCCA TCACTGGCAC TGCCTGCCTT 1981 GGGACCCGCT TCCCACCTGC TGCGGTCACC ATGGTGGCGA GCACAGCAAC CCCAGGTGTC 2041 CAGAGCACTG CCCCATGCCC ACCCTGTGTA CCCAGGTCCA GAGGGTCCGT CCACCACAGC 2101 AGCCCCAGGT GGAGGGCTGG TCTCCCTGGG GGCTCCCCAG TGGCTCTGCC CTGGCTGTGG 2161 GGGTGGAGGG ACCTTGCCAG GATGAACCCC CCAGTCCCAG GCACCCTCTA GCTCCCTCAG 2221 CCGAACAGCA CCCTGCATCT GGGGGATTGA AGCAGTCGCT GACCCCCGTC CCCAGCGGGC 2281 CCGGGCCCTC ACTCCCTGAA CCACACGGGG TTTATTTGCG GATGTTCCCT GGAGAGGTCG 2341 CTTTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2401 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2461 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCATGGGC ACGACATATC CGCGCACGCG 2521 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt