Construct: ORF TRCN0000474914
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016126.1_s317c1
- Derived from:
- ccsbBroadEn_10067
- DNA Barcode:
- GGTGTGCCTGTGAGAGTCACCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC178 (374864)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474914
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 374864 | CCDC178 | coiled-coil domain containi... | NM_001105528.3 | 99.9% | 99.7% | 1394T>A;1801G>A |
| 2 | human | 374864 | CCDC178 | coiled-coil domain containi... | NM_001308126.3 | 97.2% | 97% | 1394T>A;1801G>A;2386_2457del |
| 3 | human | 374864 | CCDC178 | coiled-coil domain containi... | NM_001371120.1 | 97.2% | 97% | 1394T>A;1801G>A;2386_2457del |
| 4 | human | 374864 | CCDC178 | coiled-coil domain containi... | NM_001371121.1 | 97.2% | 97% | 1394T>A;1801G>A;2386_2457del |
| 5 | human | 374864 | CCDC178 | coiled-coil domain containi... | NM_198995.3 | 95.5% | 95.3% | 1394T>A;1801G>A;1818_1819ins114 |
| 6 | human | 374864 | CCDC178 | coiled-coil domain containi... | XM_011525951.1 | 94.1% | 94% | 1394T>A;1801G>A;2238_2239ins150 |
| 7 | human | 374864 | CCDC178 | coiled-coil domain containi... | XM_017025724.1 | 79% | 78.9% | (many diffs) |
| 8 | human | 374864 | CCDC178 | coiled-coil domain containi... | XM_017025725.1 | 79% | 78.9% | (many diffs) |
| 9 | human | 374864 | CCDC178 | coiled-coil domain containi... | XM_011525954.2 | 54.8% | 54.7% | 1394T>A;1408_1433del;1467_1468ins1160 |
| 10 | human | 374864 | CCDC178 | coiled-coil domain containi... | XM_011525955.1 | 54.1% | 53.9% | 1394T>A;1410_1410delAins1192 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2670
- ORF length:
- 2601
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gactgaaaac aagacagttt cctcttcttc cactagagat gatcaaacca 121 atataggttt aacatgtcag gaagtaaagg ctctcagaga gaaggcatgg tcaaggacaa 181 atgaaggcaa tgccatgtct caaagtttgg ttctatatgg agcctctaag gagaacagtg 241 aaggttttca tgaaagtaaa atgacaaata ctgaaggggt gaataaaggc atttacttta 301 gctacccatg tcgacgtcac agctgtgccg tagtaaatat tccagcacct tgtgtcaaca 361 aaatgatttc acacatccaa gatgtggagt ccaaaataca ggagcatttg aaaaggtttg 421 aaacttcttt tgaagaatgg agcagaactt cttccacaaa agacctgaaa gaagattgga 481 gtgtaactac accagtgaaa gaggtcaaac caggagaaaa gagagatgaa aagtgtccag 541 agttaaagca ggaaatggaa acattgctct cagaggccat tcgtctcatt aaaagtctag 601 aaactgaccg ggcagacgct gaagaagctt taaaacaaca gagatcaaga aagaatatga 661 ttaacatgaa aattgactct tggtcagtct ggaaacttca agaactccca ttggctgtgc 721 agaaagaaca tgaggcctat ttgagtgatg ttatagaatt acaatggcat cttgaagata 781 aagctaatca actacaacat tttgaaaaac aaaagacaga gttagaagaa gcaaatgcaa 841 agattcaagc agacatagac tacatgaatg aacatggccc tctactggac tctaagcaga 901 atcaggaact tcaagatctg aagaaccatt ataaaaaaaa aatggaggta atggacctac 961 acagaaaagt taatgaagaa cttgaagaag ctttagaagc ctgtgaaaat gccagattga 1021 aggctcagca aattaaagaa gagattgata aggatattta ccaggatgaa aaaaccatag 1081 aggcctacaa gagagagata tatcaactta acagtctatt tgatcattac tcttcatcag 1141 tgataaatgt taatactaat attgaggaga aggaagagga agtgactgaa gcaataaggg 1201 aaacaaagtc atcaaaaaat gaattacatt ctctatcaaa aatgctggaa gatttgagaa 1261 gagtttatga ccaactaacc tggaagcaaa aaagtcatga aaatcagtat ctggaagcag 1321 ttaatgattt ttatgctgca aaaaaaacat gggatattga gctttctgat gttgcaaaag 1381 atttttcagc tatttctttg gcatgtacaa aactgacgga agacaataaa aaacttgaga 1441 ttgatattaa caaaataaca gaaaaaacca atgaaagcat acggaaaaaa tcaaaatacg 1501 aatctgaaat aaaatatttg acaataatga agttaaagaa tgataaacat ctcaagaaca 1561 tctataagga ggcttatcgc attggtactc ttttccacct aaccaaacac aagacagatg 1621 aaatggaaga taaaatagca gaagtgagaa gaaagttcaa gggtagagaa gaattcctga 1681 aaaaactcac tcaaggtgaa gtggctgctg gaatggtgct tcagaaaaaa ctatattcca 1741 tttacgaagt ccaggcactt gagcggaaag agcttataaa aaatagagca atatgtgcca 1801 tgtcactggc agaactacag gaacctctgc ttcaactaga agatgaagct gaaagaatca 1861 gaagtctcaa caaagaacat tctgttatgc ttaataatat aattgatcag aaagatctta 1921 ttagaagaaa ggtgggaaaa gtaaagaaaa aattacgaaa aaaggggaag aaaacccttg 1981 atgcattaat agaaactgag agtaaacgct cagcaatttt taaagaccta gaagcaacta 2041 aaagtaagac aatgattttt tatgcaaaaa taaatgaatt gaatgaggAA TTAAAAGCAA 2101 AAGAAGAAGA AAAGAAAAGT TTTGATCAGA CACTTGAAAT ATTAAAGAAC AAATTTATAA 2161 CTATGAGATT TAAAAGGGAA CATGCACAAA CTGTGTTTGA TCATTATATG CAAGAGAAAA 2221 AAGACTGTGA AGAGAGAATC TTTGAGGAAG ATCAGAGATT TAGAGTGCTC CTTGCTGTAA 2281 GACAAAAAAC TCTTCAAGAT ACCCAAAAAA TAATAGCTGA TTCACTTGAA GAAAATCTGC 2341 GTTTAGCTCA AGAGTATCAA CAGCTACAGA TTACATTCTT AAAAGAAAAG GACAATTATT 2401 TCAATATATA TGATAAACAG CTATCACTTG ATACTTCAAT TAGAGATAAG AAACAGCTCT 2461 GTCAGCTGCA GAGAAGGATG CACACACTGT GGCAGGAGCA CTTCAAACTG GTGGTCCTCT 2521 TCAGCCAGAT GAGGCTGGCC AACTTCCAGA CAGACTCTCA GGAGAGTATT CAGAAAATAT 2581 TAGCTGTGCA GGAGGAATCT TCAAATTTAA TGCAACACAT CTTAGGTTTC TTCCAGACTT 2641 TGACAGATGG CACATGCGAA AACGATGGTT TGCCAACTTT CTTGTACAAA GTGGTTGATA 2701 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2761 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGTGT 2821 GCCTGTGAGA GTCACCTACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2881 tgaaagatt