Construct: ORF TRCN0000474920
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004951.1_s317c1
- Derived from:
- ccsbBroadEn_07303
- DNA Barcode:
- AGCTCTGACTGGGTAGTAGAGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFRSF10D (8793)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474920
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8793 | TNFRSF10D | TNF receptor superfamily me... | NM_003840.5 | 99.9% | 99.7% | 929T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1224
- ORF length:
- 1158
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg actttgggga caaagcgtcc cgaccgcctc gagcgctcga gcagggcgct 121 atccaggagc caggacagcg tcgggaacca gaccatggct cctggacccc aagatcctta 181 agttcgtcgt cttcatcgtc gcggttctgc tgccggtccg ggttgactct gccaccatcc 241 cccggcagga cgaagttccc cagcagacag tggccccaca gcaacagagg cgcagcctca 301 aggaggagga gtgtccagca ggatctcata gatcagaata tactggagcc tgtaacccgt 361 gcacagaggg tgtggattac accattgctt ccaacaattt gccttcttgc ctgctatgta 421 cagtttgtaa atcaggtcaa acaaataaaa gttcctgtac cacgaccaga gacaccgtgt 481 gtcagtgtga aaaaggaagc ttccaggata aaaactcccc tgagatgtgc cggacgtgta 541 gaacagggtg tcccagaggg atggtcaagg tcagtaattg tacgccccgg agtgacatca 601 agtgcaaaaa tgaatcagct gccagttcca ctgggaaaac cccagcagcg gaggagacag 661 tgaccaccat cctggggatg cttgcctctc cctatcacta ccttatcatc atagtggttt 721 tagtcatcat tttagctgtg gttgtggttg gcttttcatg tcggaagaaa ttcatttctt 781 acctcaaagg catctgctca ggtggtggag gaggtcccga acgtgtgcac agagtccttt 841 tccggcggcg ttcatgtccT TCACGAGTTC CTGGGGCGGA GGACAATGCC CGCAACGAGA 901 CCCTGAGTAA CAGATACTTG CAGCCCACCC AGGTCTCTGA GCAGGAAATC CAAGGTCAGG 961 AGCTGGCAGA GCTAACAGGT GTGACTGTAG AGTCGCCAGA GGAGCCACAG CGTCTGCTGG 1021 AACAGGCAGA AGCTGAAGGG TGTCAGAGGA GGAGGCTGCT GGTTCCAGTG AATGACGCTG 1081 ACTCCGCTGA CATCAGCACC TTGCTGGATG CCTCGGCAAC ACTGGAAGAA GGACATGCAA 1141 AGGAAACAAT TCAGGACCAA CTGGTGGGCT CCGAAAAGCT CTTTTATGAA GAAGATGAGG 1201 CAGGCTCTGC TACGTCCTGC CTGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1261 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1321 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GCTCTGACTG 1381 GGTAGTAGAG TGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1441 att