Construct: ORF TRCN0000474922
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015712.1_s317c1
- Derived from:
- ccsbBroadEn_13458
- DNA Barcode:
- ATCACTACCCTGGTGTTATACCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PROSER2 (254427)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474922
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 254427 | PROSER2 | proline and serine rich 2 | NM_153256.4 | 78.2% | 78.1% | 732_1013del;1047C>T;1235C>T |
| 2 | human | 254427 | PROSER2 | proline and serine rich 2 | XM_011519438.2 | 78.2% | 78.1% | 732_1013del;1047C>T;1235C>T |
| 3 | human | 254427 | PROSER2 | proline and serine rich 2 | XM_017016028.2 | 78.2% | 78.1% | 732_1013del;1047C>T;1235C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1089
- ORF length:
- 1023
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgtaacccac cggaaatcag acgcatctga catgaactca gacacgtccc 121 ccagctgcag gctccgagcc ttcagcagag gcggcagcct ggagagtcga agcagcagct 181 ctcgctccag aagcttcact ttggatgatg agagcctgaa gtacctcacc catgaggaaa 241 aggatgtgct cctgtttttt gaagagacga ttgactccct agacgaggac tttgaggagc 301 cagtgctgtg cgatggagga gtgtgctgcc tctgctcccc gtctctggag gagagcacct 361 ccagtccctc cgagcctgaa gatgtcatcg acttagtgca gccagcacct ggcgccgggg 421 aagccgaggg ccttccagag gggacccagg cagcagggcc tgcacctgct gggaaggagc 481 acaggaaaca agatgctgag actcctccac ctccagaccc cccggctccc gagacccttc 541 ttgcgccacc acccctgcct agcacccccg atccccccag gagggagctg cgcgccccct 601 ccccgccggt ggagcacccc agactcctgc gcTCTGTTCC CACGCCCCTC GTTATGGCGC 661 AGAAGATTTC CGAGAGGATG GCGGGGAACG AAGCCCTCTC GCCCACCTCC CCGTTCAGGG 721 AGGGCCGGCC CGGGGAGTGG AGGACACCTG CCGCCCGGGG GCCCCGCAGT GGAGACCCTG 781 GCCCGGGGCC CAGCCACCCG GCGCTGGCCA ACGGCTTCCC AAGTGCGCAT GAGGCCCTGA 841 AGAGCGCACC CAGCTCCTTC GCGCCCGCTG GGAAGTCCCT CTGCTTCCGC CCTGGCCCGG 901 CCCTGCCCAG CACGCGGGCC CGTCAGAGCT TCCCCGGGCC CCGGCAGCCC AACGGCGCCC 961 AGGACTGGCG CCGCGCAGAC TCCCTGCCCC GGCCCCAGGG CATCACCGTG CAGTTCGTGG 1021 GCCGCGGCTC CTCGGAGGAG GCGCGCAGGG AGGCCCTGCG GAAGCTGGGG CTGCTCAGGG 1081 AGAGTTCGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1141 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1201 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCACT ACCCTGGTGT TATACCTTAC 1261 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt