Construct: ORF TRCN0000474949
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015169.1_s317c1
- Derived from:
- ccsbBroadEn_04768
- DNA Barcode:
- AATTCTCGAGTCCTTGTCACCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAA30 (122830)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474949
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122830 | NAA30 | N(alpha)-acetyltransferase ... | NM_001011713.3 | 100% | 100% | |
| 2 | mouse | 70646 | Naa30 | N(alpha)-acetyltransferase ... | NM_027492.2 | 87.1% | 86.2% | (many diffs) |
| 3 | mouse | 70646 | Naa30 | N(alpha)-acetyltransferase ... | XM_006519542.3 | 84.6% | 83.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1155
- ORF length:
- 1086
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggaggta ccgcctgggc ctagcagcct cctcccacca ccagcacctc 121 cggccccggc ggcggtcgag ccccgctgtc ccttcccggc gggggccgcc ctcgcctgct 181 gcagcgagga cgaggaggac gacgaagagc acgaaggcgg cggcagcagg agcccggcgg 241 gcggagagtc ggcgacggtg gcggccaagg ggcatccgtg cctccgctgc cctcagccgc 301 cgcaggagca gcagcagctc aacggattga ttagccccga actgcggcac ctccgggcgg 361 ccgcctccct caagagcaag gtcctgagcg tagcagaggt ggccgcgacc acagccaccc 421 ctgacggagg ccccagagcg actgcaacaa aaggagccgg ggtacactcg ggcgagaggc 481 cccctcactc cctctctagt aatgcaagaa ctgcggtccc cagcccggtg gaggcagcgg 541 cggcgagcga tcccgcggcg gcccgcaatg gactggccga gggcaccgag caggaggagg 601 aggaggaaga cgagcaggtg cggctgctgt cttcgtccct gaccgccgac tgcagcttaa 661 gaagcccttc gggcagggag gttgagcctg gggaggatcg gacgatacga tatgTCCGAT 721 ATGAATCCGA GCTACAAATG CCCGATATCA TGAGACTGAT CACCAAAGAT CTGTCCGAAC 781 CCTACTCCAT TTATACCTAT AGATATTTTA TCCACAACTG GCCACAGCTG TGCTTCTTGG 841 CCATGGTAGG GGAGGAGTGT GTAGGTGCCA TCGTTTGCAA GTTGGATATG CACAAAAAGA 901 TGTTCCGCAG AGGTTATATA GCCATGTTAG CCGTGGATTC CAAATACAGG AGAAATGGCA 961 TTGGTACTAA CTTGGTTAAG AAAGCTATAT ATGCCATGGT TGAGGGAGAC TGTGATGAGG 1021 TTGTTTTGGA AACCGAAATA ACAAATAAGT CCGCTTTGAA ACTTTATGAA AATCTTGGTT 1081 TTGTTCGAGA TAAGAGGCTG TTCAGATACT ATTTAAATGG AGTTGATGCA CTGCGACTTA 1141 AACTGTGGCT GCGTTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1201 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1261 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AATTCTCGAG TCCTTGTCAC 1321 CTCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt