Construct: ORF TRCN0000474952
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007101.1_s317c1
- Derived from:
- ccsbBroadEn_05933
- DNA Barcode:
- CGCGGTCGACCAGAGTATGATTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAPN2 (824)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474952
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 824 | CAPN2 | calpain 2 | NM_001748.5 | 99.8% | 99.8% | 66C>G;306G>A;582G>A |
| 2 | human | 824 | CAPN2 | calpain 2 | NM_001146068.1 | 88.7% | 88.7% | (many diffs) |
| 3 | human | 824 | CAPN2 | calpain 2 | XR_002957688.1 | 40.2% | (many diffs) | |
| 4 | mouse | 12334 | Capn2 | calpain 2 | NM_009794.3 | 87.6% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2166
- ORF length:
- 2100
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggcatcgcg gccaagctgg cgaaggaccg ggaggcggcc gaggggctgg 121 gctcccacga gagggccatc aagtacctca accaggacta cgaggcgctg cggaacgagt 181 gcctggaggc cgggacgctc ttccaggacc cgtccttccc ggccatcccc tcggccctgg 241 gcttcaagga gttggggccc tactccagca aaacccgggg catcgagtgg aagcgcccca 301 cggagatctg cgctgacccc cagtttatca ttggaggagc cacccgcaca gacatctgcc 361 aaggagccct aggtgactgc tggctgctgg cagccattgc ctccctcacc ttgaatgaag 421 aaatcctggc tcgagtcgtc cccctaaacc agagcttcca ggaaaactat gcagggatct 481 ttcacttcca gttctggcaa tacggcgagt gggtggaggt ggtggtggat gacaggctgc 541 ccaccaagga cggggagctg ctctttgtgc attcagccga agggagcgag ttctggagcg 601 ccctgctgga gaaggcatac gccaagatca acggatgcta tgaagcacta tcagggggtg 661 ccaccactga gggcttcgaa gacttcaccg gaggcattgc tgagtggtat gagttgaaga 721 agccccctcc caacctgttc aagatcatcc agaaagctct gcaaaaaggc tctctccttg 781 gctgctccat cgacatcacc agcgccgcgg actcggaggc catcacgttt cagaagctgg 841 tgaaggggca cgcgtactcg gtcaccggag ccgaggaggt tgaaagtaac ggaagcctac 901 agaaactgat ccgcatccga aatccctggg gagaagtgga gtggacaggg cggtggaatg 961 acaactgccc aagctggaac actatagacc cagaggagag ggaaaggctg accagacggc 1021 atgaagatgg agaattctgg atgtctttca gtgacttcct gaggcactat tcccgcctgg 1081 agatctgtaa cctgacccca gacactctca ccagcgatac ctacaagaag tggaaactca 1141 ccaaaatgga tgggaactgg aggcggggct ccaccgcggg aggttgcagg aactacccga 1201 acacattctg gatgaaccct cagtacctga tcaagctgga ggaggaggat gaggacgagg 1261 aggatgggga gagcggctgc accttcctgg tggggctcat tcagaagcac cgacggcggc 1321 agaggaagat gggcgaggac atgcacacca tcggctttgg catctatgag gttccagagg 1381 agttaagtgg gcagaccaac atccacctca gcaaaaactt cttcctgacg aatcgcgcca 1441 gggagcgctc agacaccttc atcaacctcc gggaggtgct caaccgcttc aagctgccgc 1501 caggagagta cattctcgtg ccttccacct tcgaacccaa caaggatggg gatttctgca 1561 tccgggtctt ttctgaaaag aaagctgact accaagctgt cgatgatgaa atcgaggcca 1621 atcttgaaga gttcgacatc agcgaggatg acattgatga tggattcagg agactgtttg 1681 cccagttggc aggagaggat gcggagatct ctgcctttga gctgcagacc atccTGAGAA 1741 GGGTTCTAGC AAAGCGCCAA GATATCAAGT CAGATGGCTT CAGCATCGAG ACATGCAAAA 1801 TTATGGTTGA CATGCTAGAT TCGGACGGGA GTGGCAAGCT GGGGCTGAAG GAGTTCTACA 1861 TTCTCTGGAC GAAGATTCAA AAATACCAAA AAATTTACCG AGAAATCGAC GTTGACAGGT 1921 CTGGTACCAT GAATTCCTAT GAAATGCGGA AGGCATTAGA AGAAGCAGGT TTCAAGATGC 1981 CCTGTCAACT CCACCAAGTC ATCGTTGCTC GGTTTGCAGA TGACCAGCTC ATCATCGATT 2041 TTGATAATTT TGTTCGGTGT TTGGTTCGGC TGGAAACGCT ATTCAAGATA TTTAAGCAGC 2101 TGGATCCCGA GAATACTGGA ACAATAGAGC TCGACCTTAT CTCTTGGCTC TGTTTCTCAG 2161 TACTTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2221 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2281 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGCGGTCGA CCAGAGTATG ATTGGACGCG 2341 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt