Construct: ORF TRCN0000474971

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016931.1_s317c1
Derived from:
ccsbBroadEn_00723
DNA Barcode:
GATCACGAATTCTATTATACCTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HSD17B10 (3028)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474971
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3028 HSD17B10 hydroxysteroid 17-beta dehy... NM_004493.3 100% 100%
2 human 3028 HSD17B10 hydroxysteroid 17-beta dehy... NM_001037811.2 96.5% 96.5% 566_567ins27
3 mouse 15108 Hsd17b10 hydroxysteroid (17-beta) de... NM_016763.2 85.2% 87.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
849
ORF length:
783
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc agcagcgtgt cggagcgtga agggcctggt ggcggtaata accggaggag
121cctcgggcct gggcctggcc acggcggagc gacttgtggg gcagggagcc tctgctgtgc
181ttctggacct gcccaactcg ggtggggagg cccaagccaa gaagttagga aacaactgcg
241ttttcgcccc agccgacgtg acctctgaga aggatgtgca aacagctctg gctctagcaa
301aaggaaagtt tggccgtgtg gatGTAGCTG TCAACTGTGC AGGCATCGCG GTGGCTAGCA
361AGACGTACAA CTTAAAGAAG GGCCAGACCC ATACCTTGGA AGACTTCCAG CGAGTTCTTG
421ATGTGAATCT CATGGGCACC TTCAATGTGA TCCGCCTGGT GGCTGGTGAG ATGGGCCAGA
481ATGAACCAGA CCAGGGAGGC CAACGTGGGG TCATCATCAA CACTGCCAGT GTGGCTGCCT
541TCGAGGGTCA GGTTGGACAA GCTGCATACT CTGCTTCCAA GGGGGGAATA GTGGGCATGA
601CACTGCCCAT TGCTCGGGAT CTGGCTCCCA TAGGTATCCG GGTGATGACC ATTGCCCCAG
661GTCTGTTTGG CACCCCACTG CTGACCAGCC TCCCAGAGAA AGTGTGCAAC TTCTTGGCCA
721GCCAAGTGCC CTTCCCTAGC CGACTGGGTG ACCCTGCTGA GTATGCTCAC CTCGTACAGG
781CCATCATCGA GAACCCATTC CTCAATGGAG AGGTCATCCG GCTGGATGGG GCCATTCGTA
841TGCAGCCTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
901CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
961TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGATCAC GAATTCTATT ATACCTTTAC
1021GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)