Construct: ORF TRCN0000474971
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016931.1_s317c1
- Derived from:
- ccsbBroadEn_00723
- DNA Barcode:
- GATCACGAATTCTATTATACCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSD17B10 (3028)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474971
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3028 | HSD17B10 | hydroxysteroid 17-beta dehy... | NM_004493.3 | 100% | 100% | |
| 2 | human | 3028 | HSD17B10 | hydroxysteroid 17-beta dehy... | NM_001037811.2 | 96.5% | 96.5% | 566_567ins27 |
| 3 | mouse | 15108 | Hsd17b10 | hydroxysteroid (17-beta) de... | NM_016763.2 | 85.2% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 849
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcagcgtgt cggagcgtga agggcctggt ggcggtaata accggaggag 121 cctcgggcct gggcctggcc acggcggagc gacttgtggg gcagggagcc tctgctgtgc 181 ttctggacct gcccaactcg ggtggggagg cccaagccaa gaagttagga aacaactgcg 241 ttttcgcccc agccgacgtg acctctgaga aggatgtgca aacagctctg gctctagcaa 301 aaggaaagtt tggccgtgtg gatGTAGCTG TCAACTGTGC AGGCATCGCG GTGGCTAGCA 361 AGACGTACAA CTTAAAGAAG GGCCAGACCC ATACCTTGGA AGACTTCCAG CGAGTTCTTG 421 ATGTGAATCT CATGGGCACC TTCAATGTGA TCCGCCTGGT GGCTGGTGAG ATGGGCCAGA 481 ATGAACCAGA CCAGGGAGGC CAACGTGGGG TCATCATCAA CACTGCCAGT GTGGCTGCCT 541 TCGAGGGTCA GGTTGGACAA GCTGCATACT CTGCTTCCAA GGGGGGAATA GTGGGCATGA 601 CACTGCCCAT TGCTCGGGAT CTGGCTCCCA TAGGTATCCG GGTGATGACC ATTGCCCCAG 661 GTCTGTTTGG CACCCCACTG CTGACCAGCC TCCCAGAGAA AGTGTGCAAC TTCTTGGCCA 721 GCCAAGTGCC CTTCCCTAGC CGACTGGGTG ACCCTGCTGA GTATGCTCAC CTCGTACAGG 781 CCATCATCGA GAACCCATTC CTCAATGGAG AGGTCATCCG GCTGGATGGG GCCATTCGTA 841 TGCAGCCTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGATCAC GAATTCTATT ATACCTTTAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt