Construct: ORF TRCN0000474987
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002576.1_s317c1
- Derived from:
- ccsbBroadEn_03740
- DNA Barcode:
- CACGCAACTTTAACGTGCGTGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAR1A (56681)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474987
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56681 | SAR1A | secretion associated Ras re... | NM_001142648.2 | 100% | 100% | |
| 2 | human | 56681 | SAR1A | secretion associated Ras re... | NM_020150.5 | 100% | 100% | |
| 3 | mouse | 20224 | Sar1a | secretion associated Ras re... | NM_009120.2 | 93% | 99.4% | (many diffs) |
| 4 | mouse | 20224 | Sar1a | secretion associated Ras re... | XM_006513398.2 | 93% | 99.4% | (many diffs) |
| 5 | mouse | 20224 | Sar1a | secretion associated Ras re... | XM_006513399.2 | 93% | 99.4% | (many diffs) |
| 6 | mouse | 20224 | Sar1a | secretion associated Ras re... | XM_006513401.2 | 93% | 99.4% | (many diffs) |
| 7 | mouse | 20224 | Sar1a | secretion associated Ras re... | XM_011243389.1 | 93% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 660
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tttcatcttt gagtggatct acaatggctt cagcagtgtg ctccagttcc 121 taggactgta caagaaaTCT GGAAAACTTG TATTCTTAGG TTTGGATAAT GCAGGCAAAA 181 CCACTCTTCT TCACATGCTC AAAGATGACA GATTGGGCCA ACATGTTCCA ACACTACATC 241 CGACATCAGA AGAGCTAACA ATTGCTGGAA TGACCTTTAC AACTTTTGAT CTTGGTGGGC 301 ACGAGCAAGC ACGTCGCGTT TGGAAAAATT ATCTCCCAGC AATTAATGGG ATTGTCTTTC 361 TGGTGGACTG TGCAGATCAT TCTCGCCTCG TGGAATCCAA AGTTGAGCTT AATGCTTTAA 421 TGACTGATGA AACAATATCC AATGTGCCAA TCCTTATCTT GGGTAACAAA ATTGACAGAA 481 CAGATGCAAT CAGTGAAGAA AAACTCCGTG AGATATTTGG GCTTTATGGA CAGACCACAG 541 GAAAGGGGAA TGTGACCCTG AAGGAGCTGA ATGCTCGCCC CATGGAAGTG TTCATGTGCA 601 GTGTGCTCAA GAGGCAAGGT TACGGCGAGG GTTTCCGCTG GCTCTCCCAG TATATTGACT 661 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 721 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 781 TTTATATATC TTGTGGAAAG GACGACACGC AACTTTAACG TGCGTGCCAA CGCGTTAAGT 841 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt