Transcript: Mouse XM_011243389.1

PREDICTED: Mus musculus secretion associated Ras related GTPase 1A (Sar1a), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Sar1a (20224)
Length:
2602
CDS:
110..706

Additional Resources:

NCBI RefSeq record:
XM_011243389.1
NBCI Gene record:
Sar1a (20224)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011243389.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000437023 TGCGGATCATTCTCGTCTCAT pLKO_005 415 CDS 100% 4.950 6.930 N Sar1a n/a
2 TRCN0000437584 CGCTGGCTTTCCCAGTATATT pLKO_005 680 CDS 100% 15.000 12.000 N Sar1a n/a
3 TRCN0000100808 ACTTCAGAAGAACTGACAATT pLKO.1 287 CDS 100% 13.200 9.240 N Sar1a n/a
4 TRCN0000048084 GAATCCAAAGTTGAGCTTAAT pLKO.1 437 CDS 100% 13.200 9.240 N SAR1A n/a
5 TRCN0000300297 GAATCCAAAGTTGAGCTTAAT pLKO_005 437 CDS 100% 13.200 9.240 N SAR1A n/a
6 TRCN0000416410 GAATCCAAAGTTGAGCTTAAT pLKO_005 437 CDS 100% 13.200 9.240 N Sar1a n/a
7 TRCN0000426280 ACTTCTGGACTGATCCTATTC pLKO_005 735 3UTR 100% 10.800 7.560 N Sar1a n/a
8 TRCN0000434029 CACGTCTGTCTACGCTCATTG pLKO_005 826 3UTR 100% 10.800 7.560 N Sar1a n/a
9 TRCN0000426411 CTCTTCTTCACATGCTGAAAG pLKO_005 228 CDS 100% 10.800 7.560 N Sar1a n/a
10 TRCN0000100809 CCAGTTCCTAGGACTCTACAA pLKO.1 157 CDS 100% 4.950 3.465 N Sar1a n/a
11 TRCN0000100807 CCGTGAAATATTTGGGCTGTA pLKO.1 550 CDS 100% 4.050 2.835 N Sar1a n/a
12 TRCN0000100806 GCCAATTCTTATCTTGGGAAA pLKO.1 490 CDS 100% 4.050 2.835 N Sar1a n/a
13 TRCN0000429672 GGATTGGACAATGCGGGCAAA pLKO_005 203 CDS 100% 4.050 2.835 N Sar1a n/a
14 TRCN0000100805 GCTCTTTGTGAAAGTGGTTTA pLKO.1 2320 3UTR 100% 10.800 6.480 N Sar1a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011243389.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03740 pDONR223 100% 93% 99.4% None (many diffs) n/a
2 ccsbBroad304_03740 pLX_304 0% 93% 99.4% V5 (many diffs) n/a
3 TRCN0000474987 CACGCAACTTTAACGTGCGTGCCA pLX_317 86.8% 93% 99.4% V5 (many diffs) n/a
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