Construct: ORF TRCN0000474999
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012798.1_s317c1
- Derived from:
- ccsbBroadEn_03528
- DNA Barcode:
- TTGTCTCATCGTCAGAGCGTACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKIRIN2 (55122)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474999
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55122 | AKIRIN2 | akirin 2 | NM_018064.4 | 100% | 100% | |
| 2 | mouse | 433693 | Akirin2 | akirin 2 | NM_001007589.3 | 90.6% | 93.5% | (many diffs) |
| 3 | mouse | 433693 | Akirin2 | akirin 2 | XM_011250060.2 | 90.6% | 93.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 675
- ORF length:
- 609
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtgcggagcc actctgaaaa ggactctgga tttcgacccg ctgttgagcc 121 cggcgtcccc gaagcgcagg cgatgtgcgc cattgtcggc gcccacctcg gccgctgcct 181 ccccgttgtc ggcggccgcg gccaccgccg cctccttctc cgctgcggcc gcctcgccgc 241 agaagtatct ccgaatggag CCATCCCCCT TCGGCGACGT CTCCTCCCGC CTCACCACAG 301 AACAAATTCT GTACAACATA AAACAAGAGT ATAAACGAAT GCAGAAGAGA AGACATTTAG 361 AAACGAGTTT CCAACAGACA GATCCGTGTT GTACTTCTGA TGCACAGCCA CATGCATTTC 421 TCCTCAGTGG ACCAGCTTCA CCAGGGACTT CATCTGCAGC ATCCTCACCA TTAAAAAAAG 481 AACAGCCCTT ATTTACTCTA CGGCAGGTTG GGATGATCTG TGAACGTTTG TTGAAAGAAC 541 GTGAAGAGAA AGTTCGAGAA GAATATGAAG AAATATTGAA CACAAAACTT GCAGAACAAT 601 ATGATGCGTT TGTGAAGTTT ACGCATGATC AAATAATGCG ACGATATGGA GAACAGCCTG 661 CTAGCTATGT TTCATGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 721 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 781 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTGTCTCATC GTCAGAGCGT 841 ACTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt