Construct: ORF TRCN0000475016
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009326.1_s317c1
- Derived from:
- ccsbBroadEn_08851
- DNA Barcode:
- GGGCAATCCTAGAGCGGTCAAGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMBR1 (64327)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475016
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 64327 | LMBR1 | limb development membrane p... | NM_022458.4 | 99.9% | 99.7% | 1453G>A |
2 | human | 64327 | LMBR1 | limb development membrane p... | NM_001350953.2 | 92.2% | 91.9% | 758_880del;1576G>A |
3 | human | 64327 | LMBR1 | limb development membrane p... | NM_001363410.2 | 88.9% | 88.7% | 1224_1225ins162;1291G>A |
4 | human | 64327 | LMBR1 | limb development membrane p... | NM_001363412.2 | 83.3% | 76.1% | (many diffs) |
5 | human | 64327 | LMBR1 | limb development membrane p... | NM_001363409.2 | 82% | 81.7% | 758_880del;1347_1348ins162;1414G>A |
6 | human | 64327 | LMBR1 | limb development membrane p... | NM_001363411.2 | 74.8% | 74.6% | 0_1ins369;1084G>A |
7 | human | 64327 | LMBR1 | limb development membrane p... | NM_001350954.2 | 69.9% | 66.5% | (many diffs) |
8 | human | 64327 | LMBR1 | limb development membrane p... | NM_001350955.2 | 68.9% | 68.7% | 0_1ins456;997G>A |
9 | human | 64327 | LMBR1 | limb development membrane p... | NM_001350956.2 | 68.9% | 68.7% | 0_1ins456;997G>A |
10 | human | 64327 | LMBR1 | limb development membrane p... | NM_001363413.2 | 68.9% | 68.7% | 0_1ins456;997G>A |
11 | human | 64327 | LMBR1 | limb development membrane p... | XM_017012515.2 | 63.9% | 54% | (many diffs) |
12 | human | 64327 | LMBR1 | limb development membrane p... | NM_001350957.2 | 63.8% | 63.6% | 0_1ins369;855_856ins162;922G>A |
13 | human | 64327 | LMBR1 | limb development membrane p... | XM_005249558.2 | 61.7% | 58.7% | (many diffs) |
14 | human | 64327 | LMBR1 | limb development membrane p... | NM_001350958.2 | 57.8% | 57.7% | 0_1ins456;768_769ins162;835G>A |
15 | human | 64327 | LMBR1 | limb development membrane p... | NR_146959.1 | 30.5% | 1_215del;1668G>A;1686_4814del | |
16 | human | 64327 | LMBR1 | limb development membrane p... | XR_001744850.1 | 24.6% | (many diffs) | |
17 | human | 64327 | LMBR1 | limb development membrane p... | XR_428185.1 | 23.6% | (many diffs) | |
18 | human | 64327 | LMBR1 | limb development membrane p... | XR_002956477.1 | 18.2% | 1_190del;1643G>A;1661_8052del | |
19 | human | 64327 | LMBR1 | limb development membrane p... | XR_001744848.1 | 18.1% | (many diffs) | |
20 | human | 64327 | LMBR1 | limb development membrane p... | XR_002956481.1 | 18% | (many diffs) | |
21 | human | 64327 | LMBR1 | limb development membrane p... | XR_001744847.1 | 17.9% | (many diffs) | |
22 | human | 64327 | LMBR1 | limb development membrane p... | XR_002956482.1 | 16.5% | (many diffs) | |
23 | human | 64327 | LMBR1 | limb development membrane p... | NR_146958.2 | 16.5% | (many diffs) | |
24 | human | 64327 | LMBR1 | limb development membrane p... | XR_002956479.1 | 16.2% | (many diffs) | |
25 | human | 64327 | LMBR1 | limb development membrane p... | XR_002956480.1 | 16.2% | (many diffs) | |
26 | human | 64327 | LMBR1 | limb development membrane p... | XR_002956478.1 | 15.9% | (many diffs) | |
27 | mouse | 56873 | Lmbr1 | limb region 1 | NM_020295.3 | 89.6% | 95.9% | (many diffs) |
28 | mouse | 56873 | Lmbr1 | limb region 1 | XM_006535764.3 | 88.5% | 94.4% | (many diffs) |
29 | mouse | 56873 | Lmbr1 | limb region 1 | XM_017321034.1 | 84.7% | 90.8% | (many diffs) |
30 | mouse | 56873 | Lmbr1 | limb region 1 | XM_006535765.3 | 82.7% | 85.3% | (many diffs) |
31 | mouse | 56873 | Lmbr1 | limb region 1 | XM_011249782.2 | 82.7% | 85.3% | (many diffs) |
32 | mouse | 56873 | Lmbr1 | limb region 1 | XM_011249783.1 | 72.5% | 75.5% | (many diffs) |
33 | mouse | 56873 | Lmbr1 | limb region 1 | XM_006535767.3 | 67.5% | 72.6% | (many diffs) |
34 | mouse | 56873 | Lmbr1 | limb region 1 | XM_006535768.3 | 66.6% | 71.8% | (many diffs) |
35 | mouse | 56873 | Lmbr1 | limb region 1 | XM_006535770.3 | 63.8% | 68.3% | (many diffs) |
36 | mouse | 56873 | Lmbr1 | limb region 1 | XM_006535771.3 | 46.7% | 49.3% | (many diffs) |
37 | mouse | 56873 | Lmbr1 | limb region 1 | XM_006535772.3 | 46.4% | 49.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1536
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agggcaggac gaggtgtcgg cgcgggagca gcacttccac agccaagtgc 121 gggagtccac gatatgtttc cttctttttg ccattctcta cgttgtttcc tacttcatca 181 tcacaagata caagagaaaa tcagatgaac aagaagatga agatgccatc gtcaacagga 241 tttcgttgtt tttgagcacg ttcactctcg cagtgtcagc tggggctgtt ttgcttttac 301 ccttctcaat catcagcaat gaaatcctgc tttcttttcc tcagaactac tatattcagt 361 ggctaaatgg ctccctgatt catggtttgt ggaatcttgc ttcccttttt tccaaccttt 421 gtttatttgt attgatgccc tttgcctttt tctttctgga atcagaaggc tttgctggcc 481 tgaaaaaggg aatccgagcc cgcattttag agactttggt catgcttctt cttcttgcgt 541 tactcattct tgggatagtg tgggtagctt cagcactcat tgacaacgat gccgcaagca 601 tggaatcttt atatgatctc tgggagttct atctacccta tttatattcc tgtatatcat 661 tgatgggatg tttgttactt ctcttgtgta caccagttgg cctttctcgt atgttcacag 721 tgatgggtca gttgctagtg aagccaacaa ttcttgaaga cctggatgaa caaatttata 781 tcattacctt agaggaagaa gcactccaga gacgactaaa tgggctgtct tcatcggtgg 841 aatacaacat aatggagttg gaacaagaac ttgaaaatgt aaagactctt aagacaaaat 901 tagagaggcg aaaaaaggct tcagcatggg aaagaaattt ggtgtatccc gctgttatgg 961 ttctccttct tattgagaca tccatctcgg tcctcttggt ggcttgtaat attctttgcc 1021 tattggttga tgaaacagca atgccaaaag gaacaagggg gcctggaata ggaaatgcct 1081 ctctttctac gtttggtttt gtgggagctg cgcttgaaat cattttgatt ttctatctta 1141 tggtgtcctc tgttgtcggc ttctatagcc ttcgattttt tggaaacttt actcccaaga 1201 aagatgacac aactatgaca aagatcattg gaaattgtgt gtccatcttg gtttTGAGCT 1261 CTGCTCTGCC TGTGATGTCG AGAACACTGG GAATCACTAG ATTTGATCTA CTTGGCGACT 1321 TTGGAAGGTT TAATTGGCTG GGAAATTTCT ATATTGTATT ATCCTACAAT TTGCTTTTTG 1381 CTATTGTGAC AACATTGTGT CTGGTCCGAA AATTCACCTC TGCAGTTCGA GAAGAACTTT 1441 TCAAGGCCCT AGGGCTTCAT AAACTTCACT TACCAAATAC TTCAAGGGAT TCAGAAACAG 1501 CCAAGCCTTC TGTAAATAGG CATCAGAAAG CACTGTGCCC AACTTTCTTG TACAAAGTGG 1561 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1621 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1681 AGGGCAATCC TAGAGCGGTC AAGTGACGCG TTAAGTCgac aatcaacctc tggattacaa 1741 aatttgtgaa agatt