Construct: ORF TRCN0000475027
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003724.1_s317c1
- Derived from:
- ccsbBroadEn_02475
- DNA Barcode:
- TGCTAGCTTGATCTCTATCTACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPC2 (10577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475027
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10577 | NPC2 | NPC intracellular cholester... | NM_006432.4 | 100% | 100% | |
| 2 | human | 10577 | NPC2 | NPC intracellular cholester... | NM_001363688.1 | 86% | 85.6% | 444_446delAAGinsT;449_450insC;454_522delinsC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 519
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg tttcctggca gctacattcc tgctcctggc gcTCAGCACC GCTGCCCAGG 121 CCGAACCGGT GCAGTTCAAG GACTGCGGTT CTGTGGATGG AGTTATAAAG GAAGTGAATG 181 TGAGCCCATG CCCCACCCAA CCCTGCCAGC TGAGCAAAGG ACAGTCTTAC AGCGTCAATG 241 TCACCTTCAC CAGCAATATT CAGTCTAAAA GCAGCAAGGC CGTGGTGCAT GGCATCCTGA 301 TGGGCGTCCC AGTTCCCTTT CCCATTCCTG AGCCTGATGG TTGTAAGAGT GGAATTAACT 361 GCCCTATCCA AAAAGACAAG ACCTATAGCT ACCTGAATAA ACTACCAGTG AAAAGCGAAT 421 ATCCCTCTAT AAAACTGGTG GTGGAGTGGC AACTTCAGGA TGACAAAAAC CAAAGTCTCT 481 TCTGCTGGGA AATCCCAGTA CAGATCGTTT CTCATCTCTA CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGATGCTAG CTTGATCTCT ATCTACTAAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt