Construct: ORF TRCN0000475031
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007928.1_s317c1
- Derived from:
- ccsbBroadEn_08175
- DNA Barcode:
- TACCTGGACCTTACGTCGGACTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- G0S2 (50486)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475031
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50486 | G0S2 | G0/G1 switch 2 | NM_015714.4 | 99.6% | 100% | 297G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 375
- ORF length:
- 309
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA AACGGTCCAG GAGCTGATCC CCCTGGCCAA GGAGATGATG GCCCAGAAGC 121 GCAAGGGGAA GATGGTGAAG CTGTACGTGC TGGGCAGCGT GCTGGCCCTC TTCGGCGTGG 181 TGCTCGGCCT GATGGAGACT GTGTGCAGCC CCTTCACGGC CGCCAGACGT CTGCGGGACC 241 AGGAGGCAGC CGTGGCGGAG CTGCAGGCCG CCCTGGAGCG ACAGGCTCTC CAGAAGCAAG 301 CCCTGCAGGA GAAAGGCAAG CAGCAGGACA CGGTCCTCGG CGGCCGGGCC CTGTCCAACC 361 GACAGCACGC CTCCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 421 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 481 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TACCTGGACC TTACGTCGGA 541 CTGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt