Construct: ORF TRCN0000475033
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005972.1_s317c1
- Derived from:
- ccsbBroadEn_12656
- DNA Barcode:
- TCGACCCATGTCGCACGTAAGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOCK5 (80005)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475033
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80005 | DOCK5 | dedicator of cytokinesis 5 | NM_001322810.2 | 21.3% | 18.1% | (many diffs) |
| 2 | human | 80005 | DOCK5 | dedicator of cytokinesis 5 | NM_024940.8 | 18.6% | 18.7% | 891G>A;1050_5610delinsG |
| 3 | mouse | 68813 | Dock5 | dedicator of cytokinesis 5 | NM_177780.3 | 16.3% | 17.9% | (many diffs) |
| 4 | mouse | 68813 | Dock5 | dedicator of cytokinesis 5 | XM_011245170.2 | 15.7% | 17.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgctggatc ccgaccaaga ggcagaagta cggggttgcg atctataact 121 acaatgcttc tcaagatgtg gagctctcct tgcagatcgg tgacacagtt cacatcctgg 181 agatgtacga gggttggtac agaggatata ccctccaaaa taaatctaaa aagggcattt 241 tccctgaaac atatatccat ttgaaagagg caactgtgga agacctgggg cagcatgaaa 301 ccgtgattcc tggcgagctc cccctggtgc aggagctcac gtccactctg cgagaatggg 361 ctgtcatctg gcgaaagctc tacgtgaaca acaagctcac cctcttccgc cagctgcagc 421 agatgacgta cagcctgatc gagtggcggt cccagatcct gtctgggacg ctccccaagg 481 atgaactggc agagctcaag aagaaagtca cagccaaaat tgatcatggg aacagaatgc 541 tggggttaga tctggtggtg cgagatgaca atgggaacat cctagaccct gacgaaacca 601 gcaccattgc cctcttcaag gcccatgagg tggcctccaa aaggattgag gaaaagatcc 661 aagaagagaa gtcaatcctg cagaacctcg atttgcgggg ccagtccatc ttcagtacca 721 tccacaccTA TGGCCTCTAT GTGAACTTCA AGAACTTTGT CTGCAACATC GGGGAAGATG 781 CAGAGTTGTT TATGGCCCTC TACGACCCAG ACCAGTCCAC TTTTATCAGT GAGAACTATC 841 TAATTCGTTG GGGCAGTAAC GGGATGCCCA AGGAAATAGA GAAGCTCAAT AACCTCCAAG 901 CAGTGTTTAC AGACCTTAGC AGCATGGACC TCATCCGGCC CCGCGTCAGC CTTGTATGCC 961 AGATTGTCCG CGTGGGCCAT ATGGAGCTGA AGGAAGGCAA GAAGCACACC TGTGGACTCC 1021 GAAGACCTTT TGGAGTGGCA GTGATGGATA TTACTGATAT CATACATGGG AAGGTGGATG 1081 ATGAAGAAAA GCAGCATTTT ATTCCCTTTC AGCAGTACCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 ATCGACCCAT GTCGCACGTA AGCGCACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt