Construct: ORF TRCN0000475039
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013595.1_s317c1
- Derived from:
- BRDN0000389627
- DNA Barcode:
- AAGGTGCCAGCCCGGTGTACCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIL1 (64374)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475039
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64374 | SIL1 | SIL1 nucleotide exchange fa... | NM_001037633.2 | 99.8% | 97.8% | 2T>N;153A>G |
| 2 | human | 64374 | SIL1 | SIL1 nucleotide exchange fa... | NM_022464.5 | 99.8% | 97.8% | 2T>N;153A>G |
| 3 | human | 64374 | SIL1 | SIL1 nucleotide exchange fa... | XM_024446164.1 | 99.8% | 97.8% | 2T>N;153A>G |
| 4 | human | 64374 | SIL1 | SIL1 nucleotide exchange fa... | XM_011543570.2 | 97.7% | 95.7% | 1_30del;32T>N;183A>G |
| 5 | mouse | 81500 | Sil1 | endoplasmic reticulum chape... | NM_030749.2 | 84.2% | 83% | (many diffs) |
| 6 | mouse | 81500 | Sil1 | endoplasmic reticulum chape... | XM_006526365.3 | 84.2% | 83% | (many diffs) |
| 7 | mouse | 81500 | Sil1 | endoplasmic reticulum chape... | XM_006526366.3 | 84.2% | 83% | (many diffs) |
| 8 | mouse | 81500 | Sil1 | endoplasmic reticulum chape... | XM_011247043.2 | 84.2% | 83% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 1449
- ORF length:
- 1353
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcanggc tccccagagc ctgccttcat ctaggatggc tcctctgggc atgctgcttg 121 ggctgctgat ggccgcctgc ttcaccttct gcctcagtca tcagaacctg aaggagtttg 181 ccctgaccaa cccagagaag agcagcacca aagaaacgga gagaaaagaa accaaagccg 241 aggaggagct ggatgccgaa gtcctggagg tgttccaccc gacgcatgag tggcaggccc 301 ttcagccagg gcaggctgtc cctgcaggat cccacgtacg gctgaatctt cagactgggg 361 aaagagaggc aaaactccaa tatgaggaca agttccgaaa taatttgaaa ggcaaaaggc 421 tggatatcaa caccaacacc tacacatctc aggatctcaa gagtgcactg gcaaaattca 481 aggagggggc agagatggag agttcaaagg aagacaaggc aaggcaggct gaggtaaagc 541 ggctcttccg ccccattgag gaactgaaga aagactttga tgagctgaat gttgtcattg 601 agactgacat gcagatcatg gtacggctga tcaacaagtt caatagttcc agctccagtt 661 tggaagagaa gattgctgcg ctctttgatc ttgaatatta tgtccatcag atggacaatg 721 cgcaggacct gctttccttt ggtggtcttc aagtggtgat caatgggctg aacagcacag 781 agcccctcgt gaaggagtat gctgcgtttg tgctgggcgc tgccttttcc agcaacccca 841 aggtccaggt ggaggccatc gaagggggag ccctgcagaa gctgctggtc atcctggcca 901 cggagcagcc gctcactgca aagaagaagg tcctgtttgc actgtgctcc ctgctgcgcc 961 acttccccta tgcccagcgg cagttcctga agctcggggg gctgcaggtc ctgaggaccc 1021 tggtgcagga gaagggcacg gaggtgctcg ccgtgcgcgt ggtcacactg ctctacgacc 1081 tggtcacgga gaagatgttc gccgaggagg aggctgagct gacccaggag atgtccccag 1141 agaagctgca gcagtatcgc caggtacacc tcctgccagg cctgtgggaa cagggctggt 1201 gcgagatcac ggcccacctc ctggcgctgc ccgagcatga tgcccgtgag aaggtgctgc 1261 agacactggg cgtcctcctg accacctgcc gggaccgcta ccgtcaggac ccccagctcg 1321 gcaggacact ggccagcctg caggctgagt accaggtgct ggccagcctg gagctgcagg 1381 atggtgagga cgagggctac ttccaggagc tgctgggctc tgtcaacagc ttgctgaagg 1441 agcTGAGATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1501 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACCTGAA AGTATTTCGA 1561 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAGGTG CCAGCCCGGT GTACCTTTAC 1621 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt