Construct: ORF TRCN0000475049
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009013.1_s317c1
- Derived from:
- ccsbBroadEn_05980
- DNA Barcode:
- CGGTCAACATCTGGGGCGTAACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDKN1B (1027)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475049
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1027 | CDKN1B | cyclin dependent kinase inh... | NM_004064.4 | 99.8% | 99.4% | 326T>G |
| 2 | mouse | 12576 | Cdkn1b | cyclin-dependent kinase inh... | NM_009875.4 | 87% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 660
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aaacgtgcga gtgtctaacg ggagccctag cctggagcgg atggacgcca 121 ggcaggcgga gcaccccaag ccctcggcct gcaggaacct cttcggcccg gtggaccacg 181 aagagttaac ccgggacttG GAGAAGCACT GCAGAGACAT GGAAGAGGCG AGCCAGCGCA 241 AGTGGAATTT CGATTTTCAG AATCACAAAC CCCTAGAGGG CAAGTACGAG TGGCAAGAGG 301 TGGAGAAGGG CAGCTTGCCC GAGTTCTACT ACAGACCCCC GCGGCCCCCC AAAGGTGCCT 361 GCAAGGTGCC GGCGCAGGAG AGCCAGGATG GCAGCGGGAG CCGCCCGGCG GCGCCTTTAA 421 TTGGGGCTCC GGCTAACTCT GAGGACACGC ATTTGGTGGA CCCAAAGACT GATCCGTCGG 481 ACAGCCAGAC GGGGTTAGCG GAGCAATGCG CAGGAATAAG GAAGCGACCT GCAACCGACG 541 ATTCTTCTAC TCAAAACAAA AGAGCCAACA GAACAGAAGA AAATGTTTCA GACGGTTCCC 601 CAAATGCCGG TTCTGTGGAG CAGACGCCCA AGAAGCCTGG CCTCAGAAGA CGTCAAACGT 661 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 721 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 781 TTTATATATC TTGTGGAAAG GACGACGGTC AACATCTGGG GCGTAACCCA CGCGTTAAGT 841 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt