Transcript: Human NM_004064.4

Homo sapiens cyclin dependent kinase inhibitor 1B (CDKN1B), mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
CDKN1B (1027)
Length:
2535
CDS:
571..1167

Additional Resources:

NCBI RefSeq record:
NM_004064.4
NBCI Gene record:
CDKN1B (1027)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004064.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000356317 ACGTAAACAGCTCGAATTAAG pLKO_005 1162 CDS 100% 13.200 18.480 N CDKN1B n/a
2 TRCN0000039930 GCGCAAGTGGAATTTCGATTT pLKO.1 741 CDS 100% 10.800 15.120 N CDKN1B n/a
3 TRCN0000288586 GCGCAAGTGGAATTTCGATTT pLKO_005 741 CDS 100% 10.800 15.120 N CDKN1B n/a
4 TRCN0000039929 CCGACGATTCTTCTACTCAAA pLKO.1 1040 CDS 100% 4.950 6.930 N CDKN1B n/a
5 TRCN0000009856 GAGCAATGCGCAGGAATAAGG pLKO.1 1006 CDS 100% 4.950 6.930 N CDKN1B n/a
6 TRCN0000307475 GCTTGCCCGAGTTCTACTACA pLKO_005 818 CDS 100% 4.950 6.930 N Cdkn1b n/a
7 TRCN0000307476 GCTTGCCCGAGTTCTACTACA pLKO_005 818 CDS 100% 4.950 6.930 N CDKN1B n/a
8 TRCN0000039932 CAGCGCAAGTGGAATTTCGAT pLKO.1 739 CDS 100% 3.000 4.200 N CDKN1B n/a
9 TRCN0000009858 CACACTTGTAGGATAAGTGAA pLKO.1 2148 3UTR 100% 0.495 0.693 N CDKN1B n/a
10 TRCN0000356318 GATGTAGCATTATGCAATTAG pLKO_005 1367 3UTR 100% 13.200 10.560 N CDKN1B n/a
11 TRCN0000039928 GTAGGATAAGTGAAATGGATA pLKO.1 2155 3UTR 100% 4.950 3.960 N CDKN1B n/a
12 TRCN0000368691 CGGTGGACCACGAAGAGTTAA pLKO_005 674 CDS 100% 13.200 9.240 N CDKN1B n/a
13 TRCN0000295863 GGAATGGACATCCTGTATAAG pLKO_005 1275 3UTR 100% 13.200 9.240 N CDKN1B n/a
14 TRCN0000295862 TAACTCTGAGGACACGCATTT pLKO_005 939 CDS 100% 10.800 7.560 N CDKN1B n/a
15 TRCN0000039931 CCTCAGAAGACGTCAAACGTA pLKO.1 1146 CDS 100% 3.000 2.100 N CDKN1B n/a
16 TRCN0000009857 GAATGGTGATCACTCCAGGTA pLKO.1 1857 3UTR 100% 2.640 1.848 N CDKN1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004064.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05980 pDONR223 100% 99.8% 99.4% None 326T>G n/a
2 ccsbBroad304_05980 pLX_304 0% 99.8% 99.4% V5 326T>G n/a
3 TRCN0000475049 CGGTCAACATCTGGGGCGTAACCC pLX_317 76.7% 99.8% 99.4% V5 326T>G n/a
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