Construct: ORF TRCN0000475065
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013324.1_s317c1
- Derived from:
- ccsbBroadEn_00535
- DNA Barcode:
- ACGTTCTAGCTCTGAGCCAAAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FABP3 (2170)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475065
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2170 | FABP3 | fatty acid binding protein 3 | NM_004102.5 | 100% | 100% | |
| 2 | human | 2170 | FABP3 | fatty acid binding protein 3 | NM_001320996.1 | 92.3% | 92.3% | 73_105del |
| 3 | human | 2170 | FABP3 | fatty acid binding protein 3 | XM_011541007.3 | 91.8% | 85.5% | (many diffs) |
| 4 | mouse | 14077 | Fabp3 | fatty acid binding protein ... | NM_010174.1 | 83.4% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 465
- ORF length:
- 399
- Sequence:
-
1 tcttCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGT GGACGCTTTC CTGGGCACCT GGAAGCTAGT GGACAGCAAG AATTTCGATG 121 ACTACATGAA GTCACTCGGT GTGGGTTTTG CTACCAGGCA GGTGGCCAGC ATGACCAAGC 181 CTACCACAAT CATCGAAAAG AATGGGGACA TTCTCACCCT AAAAACACAC AGCACCTTCA 241 AGAACACAGA GATCAGCTTT AAGTTGGGGG TGGAGTTCGA TGAGACAACA GCAGATGACA 301 GGAAGGTCAA GTCCATTGTG ACACTGGATG GAGGGAAACT TGTTCACCTG CAGAAATGGG 361 ACGGGCAAGA GACCACACTT GTGCGGGAGC TAATTGATGG AAAACTCATC CTGACACTCA 421 CCCACGGCAC TGCAGTTTGC ACTCGCACTT ATGAGAAAGA GGCATGCCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA ACGTTCTAGC TCTGAGCCAA AGCGACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt