Construct: ORF TRCN0000475072
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011850.1_s317c1
- Derived from:
- ccsbBroadEn_10233
- DNA Barcode:
- TTATCTATTTAAGACCTTCGTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PART1 (25859)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475072
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25859 | PART1 | prostate androgen-regulated... | NR_028508.1 | 8.3% | 1_22del;200_2108del | |
| 2 | human | 25859 | PART1 | prostate androgen-regulated... | NR_024617.1 | 7% | 1_241del;419_2495del | |
| 3 | human | 25859 | PART1 | prostate androgen-regulated... | NR_028509.1 | 2.9% | 1_22del;200_5914del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 317
- ORF end:
- 494
- ORF length:
- 177
- Sequence:
-
1 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 61 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 121 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 181 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 241 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 301 CAAAAAAGTT GGCACCATGC AATGTCAGCT ATTTAGGACA GAAACATCCA AGGCCGTGTC 361 AGAACTCAAT TACGACTACA TATGCATTAA GGCAGGAACT GGCAGGCCTC AGGGTACGCC 421 AACTATAGGA CTCGTGCTTC TCGTACGCTG GGCTATAATC TATGAAACTG AGCTCCAGAG 481 CCAGCCAATC ACTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 541 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 601 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TATCTATTTA AGACCTTCGT 661 CTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att