Construct: ORF TRCN0000475093
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008270.1_s317c1
- Derived from:
- ccsbBroadEn_05590
- DNA Barcode:
- GCAACGCCCATGGACAGAAATTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOTCH2NLA (388677)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475093
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100996717 | NOTCH2NLC | notch 2 N-terminal like C | NM_001364012.2 | 100% | 100% | |
| 2 | human | 388677 | NOTCH2NLA | notch 2 N-terminal like A | NM_203458.4 | 100% | 100% | |
| 3 | human | 388677 | NOTCH2NLA | notch 2 N-terminal like A | NM_001364006.1 | 94.7% | 94.7% | 1_39del |
| 4 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | NM_001364008.1 | 94.5% | 94.3% | 1_39del;228A>G;512T>C |
| 5 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | NM_001364007.2 | 85.5% | 85.4% | 1_117del;306A>G;590T>C |
| 6 | human | 100996717 | NOTCH2NLC | notch 2 N-terminal like C | NM_001364013.1 | 80.5% | 80.5% | 1_171del |
| 7 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_006711700.4 | 78.1% | 78% | 1_195del;384A>G;668T>C |
| 8 | human | 101929796 | NOTCH2NLR | notch 2 N-terminal like R | NR_157049.1 | 61.8% | (many diffs) | |
| 9 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_011510256.2 | 57.1% | 57% | 1_528del;717A>G;1001T>C |
| 10 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452030.1 | 51.5% | 51.5% | (many diffs) |
| 11 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452031.1 | 51.5% | 51.5% | (many diffs) |
| 12 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452032.1 | 51.5% | 51.5% | (many diffs) |
| 13 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452035.1 | 51.5% | 51.5% | (many diffs) |
| 14 | human | 4853 | NOTCH2 | notch receptor 2 | NM_001200001.1 | 18.2% | 17.3% | (many diffs) |
| 15 | human | 4853 | NOTCH2 | notch receptor 2 | NM_024408.4 | 9.1% | 8.6% | (many diffs) |
| 16 | mouse | 18129 | Notch2 | notch 2 | NM_010928.2 | 8% | 7.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 777
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgtgttacc taccacaatg gcacaggata ctgcaaatgt ccagaaggct 121 tcttggggga atattgtcaa catcgagacc cctgtgagaa gaaccgctgc cagaatggtg 181 ggacttgtgt ggcccaggcc atgctgggga aagccacgtg ccgatgtgcc tcagggttta 241 caggagagga ctgccagtac tcgacatctc atccatgctt tgtgtctcga ccttgcctga 301 atggcggcac atgccatatg cTCAGCCGGG ATACCTATGA GTGCACCTGT CAAGTCGGGT 361 TTACAGGTAA GGAGTGCCAA TGGACCGATG CCTGCCTGTC TCATCCCTGT GCAAATGGAA 421 GTACCTGTAC CACTGTGGCC AACCAGTTCT CCTGCAAATG CCTCACAGGC TTCACAGGGC 481 AGAAGTGTGA GACTGATGTC AATGAGTGTG ACATTCCAGG ACACTGCCAG CATGGTGGCA 541 CCTGCCTCAA CCTGCCTGGT TCCTACCAGT GCCAGTGCCT TCAGGGCTTC ACAGGCCAGT 601 ACTGTGACAG CCTGTATGTG CCCTGTGCAC CCTCGCCTTG TGTCAATGGA GGCACCTGTC 661 GGCAGACTGG TGACTTCACT TTTGAGTGCA ACTGCCTTCC AGAAACAGTG AGAAGAGGAA 721 CAGAGCTCTG GGAAAGAGAC AGGGAAGTCT GGAATGGAAA AGAACACGAT GAGAATTTGC 781 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GAGCAACGCC CATGGACAGA AATTTGACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt