Construct: ORF TRCN0000475102
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018835.1_s317c1
- Derived from:
- ccsbBroadEn_15310
- DNA Barcode:
- AACTCGTTGAACAGCTTATCGAGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- BMP2KL (347359)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475102
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55589 | BMP2K | BMP2 inducible kinase | XM_017008382.1 | 44.6% | 15.9% | (many diffs) |
| 2 | human | 55589 | BMP2K | BMP2 inducible kinase | XM_017008381.1 | 35.7% | 12.7% | (many diffs) |
| 3 | human | 55589 | BMP2K | BMP2 inducible kinase | XM_005263117.1 | 34.3% | 12.2% | (many diffs) |
| 4 | human | 55589 | BMP2K | BMP2 inducible kinase | NM_198892.1 | 33.2% | 11.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 543
- ORF length:
- 474
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gattgcccct tctcctaaga gcagtgaaga ggaagggcaa aaggatgaag 121 aagttcttca gggagaacaa ggagatttta atgataatga taccgaacca gaaaatctgg 181 gtcacaggcc tctcctcatg gattctgaag atgaggaaga agaggagaaa cgtagctctg 241 attctgatta tgagcaggct aaagcaaagt acagtgatat gagccctgtc tacagagaca 301 aatctggcag tggaccaacc caagatatta atacaatact cctcacctca gcccaattgt 361 cctctgatgt tggagtggag actcctaaac aggagttcga tatatttggc gccgtcccct 421 tctttgcagt gtgtgctcaa cagccccagc aagaaaagaa tgaaaagagc ctccctcaac 481 acaggtttcc tgctacggga ctccagcagg aggaatttga tgttattcac aaaggcgcct 541 tttagcaaga aggtgaatgt acaagaatgc catgcggtgg ggcctgagac acatcccaaa 601 agcatagata tatttgactt cactccattt cagccnttcc tcacatcaac aagtaaaagt 661 gaaagcaacg aggacctttt tgggcttgtg ccctttgagg aaataatggg gagccagcag 721 caaaaagtca aaacagcgca gcttacagaa actgtcctct cgccaaaggc gcacaaagca 781 ggatatgtcc aaaagtantn ngnagcggca tcatggcacg ccaactagca aaaagnagac 841 tttgaagccn anctactacg cactccagag agggctcgca ggcacaaaaa agtggggtcg 901 ccgagtctct caaaccagca atgaatttgt aaccatctca gactccaagg agaacattgg 961 tgccgcagtg acTGATGGGA ACGATAGGGG GAACGTCTTA CAACTCGAGG AGAGTCTGTT 1021 GGACCCCTTT GGTGCCAAGC CCTTCCATCC TCCAGACCTG TCATGGCACC CTCTACATCA 1081 GGGCCTGAAT GACATCCGTG CTGATCACAA TACTGTCCTG CCTAAGCAGC CAAGGCAAAA 1141 TTCACTACAT GGGTCATTCC ATAGTGCAGA TGTATTGACA ATGGATGATT TTGGTGCCAT 1201 GCCCTTTACA GAACTTGTGG TGCAAAGCAT CACTCTACAG CAGTCCCAAC AGTCCCAACC 1261 AGTCGAATTA GACCCATTTG GTGCTGCCCC ATTTCCTTCT AAACAGTTGC CAACTTTCTT 1321 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 1381 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 1441 TGGAAAGGAC GAAACTCGTT GAACAGCTTA TCGAGGACGC GTTAAGTCga caatcaacct 1501 ctggattaca aaatttgtga aagatt