Construct: ORF TRCN0000475104
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015122.1_s317c1
- Derived from:
- ccsbBroadEn_10868
- DNA Barcode:
- CTTGATCCTTGTCTTACCGCAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GUCY1A1 (2982)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475104
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130687.2 | 22.2% | 19.9% | (many diffs) |
| 2 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_000856.6 | 20.1% | 18% | (many diffs) |
| 3 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130682.3 | 20.1% | 18% | (many diffs) |
| 4 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130683.4 | 20.1% | 18% | (many diffs) |
| 5 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130684.3 | 20.1% | 18% | (many diffs) |
| 6 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001256449.2 | 20.1% | 18% | (many diffs) |
| 7 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_005262955.3 | 20.1% | 18% | (many diffs) |
| 8 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_006714196.2 | 20.1% | 18% | (many diffs) |
| 9 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_006714197.2 | 20.1% | 18% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 519
- ORF length:
- 429
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTTCTGCAC GAAGCTCAAG GATCTCAAGA 121 TCACAGGAGA GTGTCCTTTC TCCTTACTGG CACCAGGTCA AGTTCCTAAC GAGTCTTCAG 181 AGGAGGCAGC AGGAAGCTCA GAGAGCTGCA AAGCAACCGT GCCCATCTGT CAAGACATTC 241 CTGAGAAGAA CATACAAGAA AGTCTTCCTC AAAGAAAAAC CAGTCGGAGC CGAGTCTATC 301 TTCACACTTT GGCAGAGAGT ATTTGCAAAC TGATTTTCCC AGAGTTTGAA CGGCTGAATG 361 TTGCACTTCA GAGAACATTG GCAAAGCACA AAATAAAAGA AAGCAGGAAA TCTTTGGAAA 421 GAGAAGACTT TGAAAAAACA ATTGCAGAGC AAGCAGTTGC AGCAGGTGGA GACCATTGGC 481 GATGCCTATT GTGTAGCTGG GGGATTACAC AAAGAGAGTG CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGACTTGAT CCTTGTCTTA CCGCAACGAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt