Construct: ORF TRCN0000475109
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014364.1_s317c1
- Derived from:
- ccsbBroadEn_10672
- DNA Barcode:
- CGATTTCACAGTTGGAACTCATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADORA3 (140)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475109
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57413 | TMIGD3 | transmembrane and immunoglo... | NM_001081976.2 | 78.1% | 78.1% | 1_174del |
| 2 | human | 57413 | TMIGD3 | transmembrane and immunoglo... | NM_001302680.1 | 76.5% | 69.3% | (many diffs) |
| 3 | human | 57413 | TMIGD3 | transmembrane and immunoglo... | NM_020683.7 | 59.9% | 59.9% | 1_417del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 690
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gctcttcatt ctactctcct tggctctcat ttcagatgcc atggtcatgg 121 atgaaaaggt caagagaagc tttgtgctgg acacggcttc tgccatctgc aactacaatg 181 cccactacaa gaatcacccc aaatactggt gccgaggcta tttccgtgac tactgcaaca 241 tcatcgcctt ctcccctaac agcaccaatc atgtggccct GAGGGACACA GGGAACCAGC 301 TCATTGTCAC TATGTCCTGC CTGACCAAAG AGGACACGGG CTGGTACTGG TGTGGCATCC 361 AGCGGGACTT TGCCAGGGAT GACATGGATT TTACAGAGCT GATTGTAACT GACGACAAAG 421 GAACCCTGGC CAATGACTTT TGGTCTGGGA AAGACCTATC AGGCAACAAA ACCAGAAGCT 481 GCAAGGCTCC CAAAGTTGTC CGCAAGGCTG ACCGCTCCAG GACGTCCATT CTCATCATTT 541 GCATACTGAT CACGGGTTTG GGAATCATCT CTGTAATCAG TCATTTGACC AAAAGGAGGA 601 GAAGTCAAAG GAATAGAAGG GTAGGCAACA CTTTGAAGCC CTTCTCGCGT GTCCTGACTC 661 CAAAGGAAAT GGCTCCTACT GAACAGATGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 721 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 781 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGATT 841 TCACAGTTGG AACTCATTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 901 tgaaagatt