Construct: ORF TRCN0000475120
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003770.1_s317c1
- Derived from:
- ccsbBroadEn_02118
- DNA Barcode:
- GTATTCTACCTGATAGCGCAGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOG (9241)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475120
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9241 | NOG | noggin | NM_005450.6 | 100% | 100% | |
| 2 | mouse | 18121 | Nog | noggin | NM_008711.2 | 93.8% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 762
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcgctgcccc agcctagggg tcaccctcta cgccctggtg gtggtcctgg 121 ggctgcgggc gacaccggcc ggcggccagc actatctcca catccgcccg gcacccagcg 181 acaacctgcc cctggtggac ctcatcgaac acccagaccc tatctttgac cccaaggaaa 241 aggatctgaa cgagacgctg ctgcgctcgc tgctcggggg ccactacgac ccaggcttca 301 tggccacctc gccccccgag gaccggcccg gcgggggcgg gggtgcagct gggggcgcgg 361 aggacctggc ggagctggac cagctgctgc ggcagcggcc gtcgggggcc atgccgagcg 421 agatcaaagg gctagagttc tccgagggct tggcccaggg caagaagcag cgccTAAGCA 481 AGAAGCTGCG GAGGAAGTTA CAGATGTGGC TGTGGTCGCA GACATTCTGC CCCGTGCTGT 541 ACGCGTGGAA CGACCTGGGC AGCCGCTTTT GGCCGCGCTA CGTGAAGGTG GGCAGCTGCT 601 TCAGTAAGCG CTCGTGCTCC GTGCCCGAGG GCATGGTGTG CAAGCCGTCC AAGTCCGTGC 661 ACCTCACGGT GCTGCGGTGG CGCTGTCAGC GGCGCGGGGG CCAGCGCTGC GGCTGGATTC 721 CCATCCAGTA CCCCATCATT TCCGAGTGCA AGTGCTCGTG CTACCCAACT TTCTTGTACA 781 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 841 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 901 AGGACGAGTA TTCTACCTGA TAGCGCAGAT TACGCGTTAA GTCgacaatc aacctctgga 961 ttacaaaatt tgtgaaagat t