Construct: ORF TRCN0000475131
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009132.1_s317c1
- Derived from:
- ccsbBroadEn_01321
- DNA Barcode:
- ATCCCAGGACCAGACCGACATACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMD5 (5711)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475131
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5711 | PSMD5 | proteasome 26S subunit, non... | NM_005047.4 | 100% | 100% | |
| 2 | human | 5711 | PSMD5 | proteasome 26S subunit, non... | NM_001270427.2 | 91.4% | 91.4% | 431_432ins129 |
| 3 | mouse | 66998 | Psmd5 | proteasome (prosome, macrop... | NM_080554.2 | 87.7% | 89.8% | (many diffs) |
| 4 | mouse | 66998 | Psmd5 | proteasome (prosome, macrop... | XM_006498238.3 | 57.8% | 59.1% | (many diffs) |
| 5 | mouse | 66998 | Psmd5 | proteasome (prosome, macrop... | XM_017319212.1 | 47.9% | 49% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1578
- ORF length:
- 1512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcccaggct ttggcgctgc tgagagaggt agcgaggctg gaagcgccgc 121 tggaggagct acgcgcgctt cactccgtgc tgcaggcagt gccgctcaac gagcttcgcc 181 agcaagcggc ggagctgcgc ctcggcccgc tcttctccct gcttaacgag aaccataggg 241 aaaagactac tttgtgtgta tccattctgg agagattgct ccaagctatg gaaccggttc 301 acgtggcccg gaacctcagg gttgacctgc agaggggact aattcaccct gatgattctg 361 taaaaatcct cactctttcc cagattggaa gaattgttga aaattctgat gctgttactg 421 agattctaaa taatgctgaa ttactaaaac aaattgttta ttgcattggt ggagagaatc 481 tatctgtagc aaaagcggct atcaaatccc tgtcaagaat atcactaacc caagctggac 541 tggaggcttt atttgaaagc aatctgctgg atgatttgaa aagtgtaatg aaaacaaatg 601 acattgttcg atacagggtg tatgagctaa ttatagagat ttcttccgtg tcaccagaat 661 ctttaaacta ctgtaccaca agtggattgg taacccagct cctgagagag ctgactggtg 721 aggatgtgtt ggtcagagcc acctgtatag aaatggtgac atcactggca tatactcatc 781 atgggcgaca atatcttgct caagaaggag taattgacca aatttctaat ataattgttg 841 gggcagattc agaccctttc tctagcttct atctgccagg attcgtgaag ttttttggaa 901 acctggctgt catggatagt cctcaacaga tctgtgagcg ttatcctatc tttgtggaaa 961 aagtctttga aatgatagaa agtcaggacc ccactatgat tggtgtagct gtagacacag 1021 ttggaatctt GGGATCCAAT GTTGAAGGAA AACAGGTTTT ACAGAAAACA GGAACTCGCT 1081 TTGAACGCTT GCTTATGAGA ATAGGACATC AATCAAAGAA TGCCCCAGTG GAGCTAAAAA 1141 TTAGATGTTT GGATGCAATT TCATCTCTTC TGTACTTACC ACCTGAGCAG CAGACTGATG 1201 ACCTTCTGAG GATGACAGAA TCCTGGTTTT CTTCTTTATC TCGGGATCCA CTGGAGCTCT 1261 TCCGTGGCAT TAGTAGTCAG CCCTTCCCTG AACTACACTG TGCTGCCTTA AAAGTGTTTA 1321 CGGCCATTGC AAACCAACCC TGGGCTCAGA AACTTATGTT TAACAGTCCA GGTTTTGTAG 1381 AATATGTGGT GGACCGGTCT GTGGAGCATG ACAAAGCTTC AAAGGATGCC AAATATGAAC 1441 TAGTGAAAGC ACTTGCCAAT TCCAAGACAA TTGCAGAAAT CTTTGGGAAC CCAAATTATT 1501 TGAGGCTCAG AACTTACCTG AGTGAAGGGC CATACTATGT GAAACCTGTT TCCACGACAG 1561 CAGTAGAAGG AGCCGAATGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1621 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1681 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATCCCAG GACCAGACCG 1741 ACATACGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt