Construct: ORF TRCN0000475141
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017283.1_s317c1
- Derived from:
- ccsbBroadEn_07449
- DNA Barcode:
- ACAGTTGCACCGCTCGTCTATCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RIN1 (9610)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475141
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9610 | RIN1 | Ras and Rab interactor 1 | NM_004292.3 | 99.9% | 100% | 1947A>G;2112G>A |
| 2 | human | 9610 | RIN1 | Ras and Rab interactor 1 | NM_001363559.2 | 96.2% | 96.2% | (many diffs) |
| 3 | human | 9610 | RIN1 | Ras and Rab interactor 1 | XM_017018587.1 | 92.1% | 92.2% | 1284_1285ins183;1764A>G;1929G>A |
| 4 | human | 9610 | RIN1 | Ras and Rab interactor 1 | NM_001363560.2 | 91.9% | 92% | 1284_1285ins186;1761A>G;1926G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2415
- ORF length:
- 2349
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aagccctgga gagtcaggcg cgggctctcc tggagccccc agcccgtcca 121 gcttcactac tgggcacctg gcgagagaaa agccagccca ggacccactg tatgacgtgc 181 ccaatgccag cggcgggcag gcaggcgggc cgcagcggcc ggggcgcgtt gtgagcctgc 241 gggagcgcct gctgctcacc cggcccgtgt ggctgcagct gcaagccaac gcagcggccg 301 cactgcacat gctgaggacc gagcccccgg ggacgttcct cgtgcggaaa tctaacaccc 361 gccagtgcca ggccctgtgc atgcggttgc ctgaagccag tggcccctcc ttcgtctcca 421 gccactacat cctggagagc cctggcggcg tctccttgga gggctcggag ctcatgttcc 481 cagacctagt ccagctcatc tgtgcctact gccacacccg ggacatcctt ctcctcccgc 541 tgcagctccc cagagccatc caccacgcag ccactcacaa agagctggag gccatctccc 601 atctgggcat tgagttctgg agctcctccc tcaacatcaa ggctcagcgg ggcccggctg 661 gaggcccagt gttgccccag ctgaaggccc ggtcccctca agagctggac cagggcaccg 721 gagccgcctt gtgcttcttc aaccccctgt tcccggggga cctagggccc accaagcggg 781 agaaattcaa gagaagcttc aaagtgcgcg tgtccacaga gacctccagc cccctgtctc 841 cacctgccgt gccacctccc cccgtccccg tgctgccagg ggcagtcccc agccagacag 901 agcggctgcc cccttgccag ctgctacgga gggagagctc agtggggtac cgcgtgccag 961 caggcagtgg ccctagcctt ccgcctatgc cctccctcca agaggtggac tgcggctccc 1021 ccagcagctc cgaggaggag ggggtgccag ggtcccgggg gagcccagcg acctcacccc 1081 acctgggccg ccgacgacct ctgcttcggt ccatgagcgc cgccttctgc tccctactgg 1141 caccggagcg gcaggtgggc cgggctgcgg cagcactgat gcaggaccga cacacagccg 1201 cgggccagct ggtgcaggac ctactgaccc aggtgcgggc tgggcccgag ccccaggagc 1261 tgcagggcat ccgtcaggcg ctgagccggg cccgggccat gctgagtgcg gagctgggcc 1321 ctgagaagct gctgtcgcct aagaggctgg aacatgtcct ggagaagtca ttgcattgct 1381 ctgtgctcaa gcctctccgg cccatcctgg cagcccgcct gcggcgccgg cttgccgcag 1441 acggctccct gggccgccta gctgagggcc tccgcctggc ccgggcccag ggccccggag 1501 ccttcgggtc ccacctgagc ctgccctccc cagtagagtt ggagcaagtg cgccagaagc 1561 tgctgcagct gctccgcacc tactcaccca gcgcccaggt caagcggctc ctgcaggcct 1621 gcaagctgct ctacatggcc ctgaggaccc aggaagggga gggcgcgggt gccgacgagt 1681 tcctgcctct gctgagcctc gtcttggccc actgtgacct tcctgagctg ctgctggagg 1741 ccgagtacat gtcggagctg ctggagccca gcctgcttac tggagagggt ggctactacc 1801 tgaccagcct ctctgccagc ctggccctgc tgagtggcct gggtcaggcc cacaccctcc 1861 cactgagccc cgtgcaggag ctacggcgct ccctcagcct ctgggagcag cgccgcctgc 1921 ctgccaccca ctgcttccag cacctcctcc gagtagccta tcaggatccc agcagtggct 1981 gcacctccaa gaccctggcc gtgcccccag aggcctcgat tgccaccctg aaccagctct 2041 gtgccaccaa gttccgagtg acccagccca acacttttgg cctcttcctg tacaaggagc 2101 agggctacca ccgcctgccc cctggggccc tggcccacag gctgcccacc actggctacc 2161 tcgtctaccg ccgggcagag tggcctgaga cccagggggc tgtgacagag gaggagggca 2221 gtgggcagtc agaggcaaga agcagagggg aggagcaagg gtgccaggga gatggggatg 2281 ctggggtcaa agccagcccc agggacattc gggaacagtc TGAGACAACT GCTGAAGGGG 2341 GCCAGGGTCA AGCCCAGGAA GGCCCTGCTC AGCCAGGGGA ACCAGAGGCA GAGGGAAGCC 2401 GGGCAGCAGA GGAGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2461 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2521 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACAGTTGCAC CGCTCGTCTA 2581 TCGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt