Construct: ORF TRCN0000475142
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015014.1_s317c1
- Derived from:
- ccsbBroadEn_13035
- DNA Barcode:
- ACGCTTGATGGACTCACCCTGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PKD1L2 (114780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475142
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114780 | PKD1L2 | polycystin 1 like 2 (gene/p... | NM_001278423.2 | 99.8% | 99.6% | 77T>C |
| 2 | human | 114780 | PKD1L2 | polycystin 1 like 2 (gene/p... | NM_001076780.2 | 30.8% | 30.7% | 1_2055del;2132T>C |
| 3 | human | 114780 | PKD1L2 | polycystin 1 like 2 (gene/p... | NM_001278425.2 | 16.7% | 16.2% | (many diffs) |
| 4 | human | 114780 | PKD1L2 | polycystin 1 like 2 (gene/p... | NM_052892.4 | 12.1% | 11.7% | (many diffs) |
| 5 | human | 114780 | PKD1L2 | polycystin 1 like 2 (gene/p... | NR_126532.2 | 11.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 984
- ORF length:
- 918
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggaggactct ccagtggcta tgttcagctg gtatttggac aacaccccaa 121 cagagcaggc tgagcccctc ccggatgcct gcagactcag aggattttgg ccaaggtcct 181 taaccctcct ccagagcaac acctccacgt tgctgttgaa cagctcgttt ctgcagtccc 241 ggggagaggt catccgaatc agagccacag cactgaccag gcatgcctat ggggaggaca 301 cctatgtgat cagcactgtg cctccccgtg aggtgcctgc ctgcactatt gccccagagg 361 agggcaccgt tctgacgagc tttgccatct tctgcaacgc ctccacagcc ctgggacccc 421 tggagttctg cttctgtctg gaatcaggtt cctgcctaca ctgtggccct gaacctgccc 481 tcccatcagt gtatctgcca cttggAGAGG AGAACAATGA CTTTGTGCTG ACAGTAGTTA 541 TTTCTGCCAC CAATCGTGCA GGGGACACGC AGCAGACCCA GGCCATGGCT AAGGTGGCAC 601 TCGGAGACAC ATGTGTTGAG GATGTAGCAT TCCAGGCTGC CGTGTCAGAG AAAATCCCCA 661 CAGCTCTGCA AGGCGAGGGT GGCCCCGAGC AGCTCCTCCA GCTGGCCAAG GCTGTGTCCT 721 CCATGCTGAA CCAAGAGCAT GAAAGCCAGG GCTCAGGACA GTCACTGAGC ATAGACGTCA 781 GACAGAAGGT CAGAGAGCAT GTGCTGGGAT CACTGTCTGC AGTCACCACC GGCTTGGAGG 841 ACGTGCAGAG GGTGCAGGAG CTGGCCGAGG TGCTGAGAGA GGTGACCTGC CGGAGTAAGG 901 AACTCACACC CTCGGCCCAG GGGTCCTGCA TGGGCGATTC ATGGGAAGGT GCCCCTCCTG 961 CTGCCCATGT ATCTCACGCT AGGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1021 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1081 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CGCTTGATGG 1141 ACTCACCCTG TCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1201 att