Construct: ORF TRCN0000475153
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007375.1_s317c1
- Derived from:
- ccsbBroadEn_11433
- DNA Barcode:
- TCTCTACAACCATGATTTCAAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD302 (9936)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475153
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9936 | CD302 | CD302 molecule | NM_001198764.1 | 87.1% | 87.1% | 1_75del |
| 2 | human | 9936 | CD302 | CD302 molecule | NM_014880.5 | 73.2% | 73.2% | 1_186del |
| 3 | human | 9936 | CD302 | CD302 molecule | NM_001198763.1 | 48.2% | 47.8% | 1_186del;295_296ins174 |
| 4 | human | 100526664 | LY75-CD302 | LY75-CD302 readthrough | NM_001198760.1 | 9.3% | 9.3% | 1_4941del |
| 5 | human | 100526664 | LY75-CD302 | LY75-CD302 readthrough | NM_001198759.1 | 9% | 9% | 1_5109del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat aagcatacat aatgaagaag aaaatgcttt tatactggat actttgaaaa 121 agcaatggaa aggcccagat gatatcctac taggcatgtt ttatgacaca gatgatgcga 181 gtttcaagtg gtttGATAAT TCAAATATGA CATTTGATAA GTGGACAGAC CAAGATGATG 241 ATGAGGATTT AGTTGACACC TGTGCTTTTC TGCACATCAA GACAGGTGAA TGGAAAAAAG 301 GAAATTGTGA AGTTTCTTCT GTGGAAGGAA CACTATGCAA AACAGCTATC CCATACAAAA 361 GGAAATATTT ATCAGATAAC CACATTTTAA TATCAGCATT GGTGATTGCT AGCACGGTAA 421 TTTTGACAGT TTTGGGAGCA ATCATTTGGT TCCTGTACAA AAAACATTCT GATTCTCGTT 481 TCACCACAGT TTTTTCAACC GCACCCCAAT CACCTTATAA TGAAGACTGT GTTTTGGTAG 541 TTGGAGAAGA AAATGAATAT CCTGTTCAAT TTGACTACCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 ATCTCTACAA CCATGATTTC AAAAGACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt