Construct: ORF TRCN0000475159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017219.1_s317c1
- Derived from:
- ccsbBroadEn_02892
- DNA Barcode:
- GCAATGTCCTAGTCAAAGCCACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM87A (25963)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475159
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25963 | TMEM87A | transmembrane protein 87A | NM_001110503.2 | 100% | 100% | |
| 2 | human | 25963 | TMEM87A | transmembrane protein 87A | XM_011521444.3 | 50% | 47.8% | (many diffs) |
| 3 | human | 25963 | TMEM87A | transmembrane protein 87A | NM_015497.5 | 31.9% | 30.5% | (many diffs) |
| 4 | human | 25963 | TMEM87A | transmembrane protein 87A | XM_005254287.2 | 31.9% | 30.5% | (many diffs) |
| 5 | human | 25963 | TMEM87A | transmembrane protein 87A | NM_001286487.1 | 21% | 18.3% | (many diffs) |
| 6 | human | 25963 | TMEM87A | transmembrane protein 87A | XM_011521443.3 | 20.9% | 18.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggctgcg tggcttcagg tgttgcctgt cattcttctg cttctgggag 121 ctcacccgtc accactgtcg tttttcagtg cgggaccggc aaccgtagct gctgccgacc 181 ggtccaaatg gcacattccg ataccgtcgg ggaaaaatta ttttagtttt ggaaagatcc 241 tcttcagaaa taccactatc ttcctgaagt ttgatggaga accTTGTGAC CTGTCTTTGA 301 ATATAACCTG GTATCTGAAA AGCGCTGATT GTTACAATGA AATCTATAAC TTCAAGGCAG 361 AAGAAGTAGA GTTGTATTTG GAAAAACTTA AGGAAAAAAG AGGCTTGTCT GGGAAATATC 421 AAACATCATC AAAATTGTTC CAGAACTGCA GTGAACTCTT TAAAACACAG ACCTTTTCTG 481 GAGATTTTAT GCATCGACTG CCTCTTTTAG GAGAAAAACA GGAGGCTAAG GAGAATGGAA 541 CAAACCTTAC CTTTATTGGA GACAAAACCA TTCAGATGCC TTTCTTGAAG AAACATTTCT 601 TGGATTGTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCAATG TCCTAGTCAA AGCCACCTAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt