Construct: ORF TRCN0000475163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014937.1_s317c1
- Derived from:
- ccsbBroadEn_09206
- DNA Barcode:
- TGATTCCGGCCTCAATAGGTTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZGPAT (84619)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475163
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84619 | ZGPAT | zinc finger CCCH-type and G... | NM_001195654.1 | 99.9% | 99.8% | 1210G>A |
| 2 | human | 84619 | ZGPAT | zinc finger CCCH-type and G... | NM_001083113.1 | 98.1% | 98% | 844_870del;1237G>A |
| 3 | human | 84619 | ZGPAT | zinc finger CCCH-type and G... | NM_001195653.1 | 98.1% | 98% | 844_870del;1237G>A |
| 4 | human | 84619 | ZGPAT | zinc finger CCCH-type and G... | NM_181485.2 | 98.1% | 98% | 844_870del;1237G>A |
| 5 | human | 84619 | ZGPAT | zinc finger CCCH-type and G... | NM_032527.4 | 94.4% | 94.3% | 844_930del;1297G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1572
- ORF length:
- 1506
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgaggagagc ctggagtcgg ccttgcagac ctaccgtgcg cagctgcagc 121 aggtggagct ggccttgggc gccggcctgg attcgtctga gcaggctgac ctgcgccagc 181 tgcaggggga cctgaaggag ctcatcgagc tcaccgaggc cagcctggtg tctgtcagga 241 agagcagctt gttggccgcg ctggacgaag agcgcccggg ccgccaggaa gatgctgagt 301 accaggcttt ccgggaggcc atcactgagg cggtggaggc accagcagcg gcccgtgggt 361 ccggatcaga gaccgttcct aaagcagagg cggggccaga atctgcggca ggtgggcagg 421 aggaggaaga gggagaggac gaggaagagc tgagtgggac aaaggtgagc gcgccctact 481 acagctcctg gggcactctg gagtatcaca acgccatggt ggtgggaacg gaagaggcgg 541 aggatggctc ggcgggtgtc cgtgtgcttt acctgtaccc cactcacaag tctctgaagc 601 cgtgcccgtt cttcctggag ggaaagtgcc gctttaagga gaactgcagg ttctcccatg 661 ggcaggtggt ctctctggat gagctgcgcc ccttccagga cccagacctg agctccctgc 721 aggccggctc tgcgtgtctg gccaagcacc aggatggcct ctggcacgca gcacgcatca 781 ccgatgtgga caacggctac tacacagtca agtttgactc gctgctgctg agggaggccg 841 tggtggaggg ggacggcatc ctgcccccac tgcgcacaga ggccacagag tccgactcag 901 acagcgacgt ggtggggtca gatgctgtgg actctgggac ctgcagctct gcctttgctg 961 gctgggaggt gcacacgcga gGTATAGGCT CCAGACTCCT CACCAAGATG GGCTATGAGT 1021 TTGGCAAGGG TTTGGGCCGA CACGCGGAAG GCCGGGTGGA GCCCATCCAT GCTGTGGTGT 1081 TGCCTCGAGG GAAGTCGCTG GACCAGTGTG TGGAGACCCT GCAGAAGCAG ACCAGGGTTG 1141 GCAAGGCTGG CACCAACAAG CCCCCCAGGT GCCGGGGAAG AGGGGCCAGG CCTGGGGGCC 1201 GCCCAGCTCC TCGGAATGTG TTTGACTTCC TCAATGAAAA GCTGCAAGGT CAGGCTCCTG 1261 GGGCCCTAGA AGCCAGGGCG GCCCCAGCGG GGAGGAGGAG CAAGGACATG TACCATGCCA 1321 GCAAGAGTGC CAAGCGGGCC CTGAGCCTGC GGCTCTTCCA GACTGAGGAG AAGATCGAGC 1381 GAACCCAGCG GGACATCAGG AGCATCCAGG AGGCTCTCGC CCGCAACGCT GGCCGGCATA 1441 GCGTGGCGTC AGCCCAGCTG CAGGAGAAGC TGGCAGGAGC CCAGCGCCAG CTGGGGCAGC 1501 TCCGGGCTCA GGAAGCCGGC CTGCAGCAGG AGCAGAGGAA GGCAGACACC CACAAGAAGA 1561 TGACTGAGTT CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1621 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTTG GCTTTATATA TCTTGTGGAA 1681 AGGACGATGA TTCCGGCCTC AATAGGTTCT AACGCGTTAA GTCgacaatc aacctctgga 1741 ttacaaaatt tgtgaaagat t