Transcript: Human NM_001195653.1

Homo sapiens zinc finger CCCH-type and G-patch domain containing (ZGPAT), transcript variant 5, mRNA.

Source:
NCBI, updated 2018-11-11
Taxon:
Homo sapiens (human)
Gene:
ZGPAT (84619)
Length:
1949
CDS:
171..1706

Additional Resources:

NCBI RefSeq record:
NM_001195653.1
NBCI Gene record:
ZGPAT (84619)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001195653.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000236465 GCTACTACACAGTCAAGTTTG pLKO_005 901 CDS 100% 10.800 15.120 N ZGPAT n/a
2 TRCN0000165398 CAACGGCTACTACACAGTCAA pLKO.1 896 CDS 100% 4.950 6.930 N ZGPAT n/a
3 TRCN0000236464 GAGGGAAAGTGCCGCTTTAAG pLKO_005 723 CDS 100% 13.200 9.240 N ZGPAT n/a
4 TRCN0000236466 CCAAGATGGGCTATGAGTTTG pLKO_005 1135 CDS 100% 10.800 7.560 N ZGPAT n/a
5 TRCN0000236463 GAAGAGCTGAGTGGGACAAAG pLKO_005 549 CDS 100% 10.800 7.560 N ZGPAT n/a
6 TRCN0000162078 CTATGAGTTTGGCAAGGGTTT pLKO.1 1145 CDS 100% 4.050 2.835 N ZGPAT n/a
7 TRCN0000166011 GCTATGAGTTTGGCAAGGGTT pLKO.1 1144 CDS 100% 2.640 1.848 N ZGPAT n/a
8 TRCN0000166186 CACAGTCAAGTTTGACTCGCT pLKO.1 908 CDS 100% 0.066 0.046 N ZGPAT n/a
9 TRCN0000158740 GTGTTTGACTTCCTCAATGAA pLKO.1 1350 CDS 100% 5.625 3.375 N ZGPAT n/a
10 TRCN0000159860 GAATGTGTTTGACTTCCTCAA pLKO.1 1346 CDS 100% 4.050 2.430 N ZGPAT n/a
11 TRCN0000160422 CAAGAAGATGACTGAGTTCTA pLKO.1 1685 CDS 100% 4.950 2.475 Y ZGPAT n/a
12 TRCN0000162675 CCACAAGAAGATGACTGAGTT pLKO.1 1682 CDS 100% 4.950 2.475 Y ZGPAT n/a
13 TRCN0000236462 CCACAAGCACTATGGACGAAG pLKO_005 1712 3UTR 100% 4.050 2.025 Y ZGPAT n/a
14 TRCN0000166702 CCCACAAGAAGATGACTGAGT pLKO.1 1681 CDS 100% 2.640 1.320 Y ZGPAT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001195653.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15198 pDONR223 89.8% 99.4% 98.8% None (many diffs) n/a
2 ccsbBroad304_15198 pLX_304 0% 99.4% 98.8% V5 (many diffs) n/a
3 TRCN0000481245 ATGTCGACGATTCAATCGATCGAT pLX_317 27.1% 99.4% 98.8% V5 (many diffs) n/a
4 ccsbBroadEn_09206 pDONR223 100% 98.1% 98% None 844_870del;1237G>A n/a
5 ccsbBroad304_09206 pLX_304 0% 98.1% 98% V5 844_870del;1237G>A n/a
6 TRCN0000475163 TGATTCCGGCCTCAATAGGTTCTA pLX_317 39.3% 98.1% 98% V5 844_870del;1237G>A n/a
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