Construct: ORF TRCN0000475175
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011316.1_s317c1
- Derived from:
- ccsbBroadEn_07255
- DNA Barcode:
- CTCCAGAGTATTAAAGAACATGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPZ (8532)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475175
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8532 | CPZ | carboxypeptidase Z | NM_001014448.2 | 99.8% | 99.8% | 188C>T;963T>C |
| 2 | human | 8532 | CPZ | carboxypeptidase Z | NM_003652.3 | 80.2% | 80.1% | 1_378del;566C>T;1341T>C |
| 3 | human | 8532 | CPZ | carboxypeptidase Z | NM_001014447.3 | 78.7% | 78.6% | (many diffs) |
| 4 | mouse | 242939 | Cpz | carboxypeptidase Z | NM_153107.2 | 64.9% | 66.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1611
- ORF length:
- 1545
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctggccctac ttccttgact gccaccgcta cttcacgaga gaggacgagg 121 gctgctatga cccgctggag aagcttcggg gaggcctgga ggctgacgag gcactgccct 181 cagggctgcc gcccaccttc atccgcttca gccaccactc ctacgcccag atggtgcgtg 241 tgctgaggcg gatggcctcc cgctgcgccc acgtggccag gacctacagc atcgggcgca 301 gcttcgacgg cagggagctg ctggtcatcg agttctccag ccgccccggc cagcacgagc 361 tgatggagcc cgaggtgaag ctcatcggca acattcatgg caacgaggtg gcgggccggg 421 agatgctcat ctacctagcc cagtacctgt gctctgagta cctgcttggt aacccccgca 481 tccagcgcct gctcaacacc acccgcatcc acctgctgcc ctccatgaac cctgacggct 541 atgaggtggc agctgccgag ggtgccggct acaacgggtg gacgagcggg aggcagaacg 601 cgcagaacct ggatctgaac cgaaatttcc cggacctgac gtccgagtac taccggctgg 661 cggagacccg cggcgcacgc agcgaccaca tccccatccc ccagcactac tggtggggta 721 aggtggcccc ggagacaaag gcaatcatga agtggatgca gaccataccc tttgtgctct 781 cagccagcct tcatgggggc gacctggtgg tgtcctaccc cttcgacttc tccaagcacc 841 cccaggagga gaagatgttt tctcccacgc ccgacgagaa gatgttcaag ctgctgtcca 901 gagcctacgc tgacgtccac cccatgatga tggacaggtc ggagaatagg tgtggaggca 961 atttcctgaa gagggggagc atcatcaacg gggcggactg gtacagcttc acgggaggca 1021 tgtccgactt caactacctg cacaccaact gctttgagat cacggtagag ctgggctgtg 1081 tgaagttccc ccccgaggag gccctgtaca cactctggca gcacaacaag gagtcactcc 1141 tgaatttcgt ggagacggtg caccggggca tcaaaggtgt ggtgacagat aaattcggca 1201 agccagtcaa aaacgcccgg atctcagtca aaggcattcg ccacgacatc accacagccc 1261 cagatggtga ctactggaga ctgctgcccc caggtatcca cattgtcatt gcccaagccc 1321 ctggctacgc caaagtcatc aagaaagtca tcatccccgc ccGGATGAAG AGGGCTGGCC 1381 GTGTGGACTT CATTCTGCAA CCTCTGGGGA TGGGACCCAA GAACTTTATT CATGGGCTGC 1441 GGAGGACTGG GCCCCACGAC CCGCTGGGAG GTGCCAGCTC TTTGGGGGAG GCCACGGAGC 1501 CCGACCCGCT CCGGGCGCGC AGGCAGCCCT CGGCCGACGG GAGTAAGCCC TGGTGGTGGT 1561 CCTACTTCAC ATCGCTGAGC ACCCACAGGC CACGCTGGCT GCTCAAGTAC TACCCAACTT 1621 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1681 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1741 CTTGTGGAAA GGACGACTCC AGAGTATTAA AGAACATGGG ACGCGTTAAG TCgacaatca 1801 acctctggat tacaaaattt gtgaaagatt