Construct: ORF TRCN0000475175

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011316.1_s317c1
Derived from:
ccsbBroadEn_07255
DNA Barcode:
CTCCAGAGTATTAAAGAACATGGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CPZ (8532)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475175
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8532 CPZ carboxypeptidase Z NM_001014448.2 99.8% 99.8% 188C>T;963T>C
2 human 8532 CPZ carboxypeptidase Z NM_003652.3 80.2% 80.1% 1_378del;566C>T;1341T>C
3 human 8532 CPZ carboxypeptidase Z NM_001014447.3 78.7% 78.6% (many diffs)
4 mouse 242939 Cpz carboxypeptidase Z NM_153107.2 64.9% 66.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1611
ORF length:
1545
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ctggccctac ttccttgact gccaccgcta cttcacgaga gaggacgagg
121gctgctatga cccgctggag aagcttcggg gaggcctgga ggctgacgag gcactgccct
181cagggctgcc gcccaccttc atccgcttca gccaccactc ctacgcccag atggtgcgtg
241tgctgaggcg gatggcctcc cgctgcgccc acgtggccag gacctacagc atcgggcgca
301gcttcgacgg cagggagctg ctggtcatcg agttctccag ccgccccggc cagcacgagc
361tgatggagcc cgaggtgaag ctcatcggca acattcatgg caacgaggtg gcgggccggg
421agatgctcat ctacctagcc cagtacctgt gctctgagta cctgcttggt aacccccgca
481tccagcgcct gctcaacacc acccgcatcc acctgctgcc ctccatgaac cctgacggct
541atgaggtggc agctgccgag ggtgccggct acaacgggtg gacgagcggg aggcagaacg
601cgcagaacct ggatctgaac cgaaatttcc cggacctgac gtccgagtac taccggctgg
661cggagacccg cggcgcacgc agcgaccaca tccccatccc ccagcactac tggtggggta
721aggtggcccc ggagacaaag gcaatcatga agtggatgca gaccataccc tttgtgctct
781cagccagcct tcatgggggc gacctggtgg tgtcctaccc cttcgacttc tccaagcacc
841cccaggagga gaagatgttt tctcccacgc ccgacgagaa gatgttcaag ctgctgtcca
901gagcctacgc tgacgtccac cccatgatga tggacaggtc ggagaatagg tgtggaggca
961atttcctgaa gagggggagc atcatcaacg gggcggactg gtacagcttc acgggaggca
1021tgtccgactt caactacctg cacaccaact gctttgagat cacggtagag ctgggctgtg
1081tgaagttccc ccccgaggag gccctgtaca cactctggca gcacaacaag gagtcactcc
1141tgaatttcgt ggagacggtg caccggggca tcaaaggtgt ggtgacagat aaattcggca
1201agccagtcaa aaacgcccgg atctcagtca aaggcattcg ccacgacatc accacagccc
1261cagatggtga ctactggaga ctgctgcccc caggtatcca cattgtcatt gcccaagccc
1321ctggctacgc caaagtcatc aagaaagtca tcatccccgc ccGGATGAAG AGGGCTGGCC
1381GTGTGGACTT CATTCTGCAA CCTCTGGGGA TGGGACCCAA GAACTTTATT CATGGGCTGC
1441GGAGGACTGG GCCCCACGAC CCGCTGGGAG GTGCCAGCTC TTTGGGGGAG GCCACGGAGC
1501CCGACCCGCT CCGGGCGCGC AGGCAGCCCT CGGCCGACGG GAGTAAGCCC TGGTGGTGGT
1561CCTACTTCAC ATCGCTGAGC ACCCACAGGC CACGCTGGCT GCTCAAGTAC TACCCAACTT
1621TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1681CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1741CTTGTGGAAA GGACGACTCC AGAGTATTAA AGAACATGGG ACGCGTTAAG TCgacaatca
1801acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)