Construct: ORF TRCN0000475176
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000111.1_s317c1
- Derived from:
- ccsbBroadEn_05992
- DNA Barcode:
- CTTGGATCGTACGGACGAATAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHI3L1 (1116)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475176
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1116 | CHI3L1 | chitinase 3 like 1 | NM_001276.4 | 99.8% | 99.7% | 433A>G;1092T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1215
- ORF length:
- 1149
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tgtgaaggcg tctcaaacag gctttgtggt cctggtgctg ctccagtgct 121 gctctgcata caaactggtc tgctactaca ccagctggtc ccagtaccgg gaaggcgatg 181 ggagctgctt cccagatgcc cttgaccgct tcctctgtac ccacatcatc tacagctttg 241 ccaatataag caacgatcac atcgacacct gggagtggaa tgatgtgacg ctctacggca 301 tgctcaacac actcaagaac aggaacccca acctgaagac tctcttgtct gtcggaggat 361 ggaactttgg gtctcaaaga ttttccaaga tagcctccaa cacccagagt cgccggactt 421 tcatcaagtc agtaccgcca tttctgcgca cccatggctt tgatgggctg gaccttgcct 481 ggctctaccc tggacgggga gacaaacagc attttaccac cctaatcaag gaaatgaagg 541 ccgaatttat aaaggaagcc cagccaggga aaaagcagct cctgctcagc gcagcactgt 601 ctgcggggaa ggtcaccatt gacagcagct atgacattgc caagatatcc caacacctgg 661 atttcattag catcatgacc tacgattttc atggagcctg gcgtgggacc acaggccatc 721 acagtcccct gttccgaggt caggaggatg caagtcctga cagattcagc aacactgact 781 atgctgtggg gtacatgttg aggctggggg ctcctgccag taagctggtg atgggcatcc 841 ccaccTTCGG GAGGAGCTTC ACTCTGGCTT CTTCTGAGAC TGGTGTTGGA GCCCCAATCT 901 CAGGACCGGG AATTCCAGGC CGGTTCACCA AGGAGGCAGG GACCCTTGCC TACTATGAGA 961 TCTGTGACTT CCTCCGCGGA GCCACAGTCC ATAGAATCCT CGGCCAGCAG GTCCCCTATG 1021 CCACCAAGGG CAACCAGTGG GTAGGATACG ACGACCAGGA AAGCGTCAAA AGCAAGGTGC 1081 AGTACCTGAA GGACAGGCAG CTGGCGGGCG CCATGGTATG GGCCCTGGAC CTGGATGACT 1141 TCCAGGGCTC CTTCTGCGGC CAGGATCTGC GCTTCCCTCT CACCAATGCC ATCAAGGATG 1201 CACTCGCTGC AACGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1261 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1321 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTTGGATCGT ACGGACGAAT 1381 AATTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt