Construct: ORF TRCN0000475191
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009393.1_s317c1
- Derived from:
- ccsbBroadEn_12308
- DNA Barcode:
- CTTGTTGACGTACAAACAAGAGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBQLN4 (56893)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475191
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56893 | UBQLN4 | ubiquilin 4 | NM_001304342.2 | 10.6% | 10.6% | 1_1557del |
| 2 | human | 56893 | UBQLN4 | ubiquilin 4 | NM_020131.5 | 10.3% | 10.3% | 1_1617del |
| 3 | human | 56893 | UBQLN4 | ubiquilin 4 | XR_921895.3 | 5.6% | 1_1413del;1600_3279del | |
| 4 | mouse | 94232 | Ubqln4 | ubiquilin 4 | XM_006502396.2 | 10.2% | 10.8% | (many diffs) |
| 5 | mouse | 94232 | Ubqln4 | ubiquilin 4 | NM_033526.2 | 9.3% | 9.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 212
- ORF end:
- 398
- ORF length:
- 186
- Sequence:
-
1 TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC 61 TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG 121 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA 181 TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGATCCAG CTTTTGGCTG GAAGTGGAAA 241 CTCACAGGTG CAGACGCCAG AAGTGAGATT TCAGCAGCAG CTGGAGCAGC TCAACTCCAT 301 GGGCTTCATC AATCGTGAGG CTAACCTGCA GGCCCTGATT GCCACAGGAG GGGACATCAA 361 CGCAGCTATC GAGAGACTGC TGGGCTCCCA GCTCTCCTAC CCAACTTTCT TGTACAAAGT 421 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 481 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 541 CGACTTGTTG ACGTACAAAC AAGAGTAACG CGTTAAGTCg acaatcaacc tctggattac 601 aaaatttgtg aaagatt