Construct: ORF TRCN0000475224
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003914.1_s317c1
- Derived from:
- ccsbBroadEn_12011
- DNA Barcode:
- GACGTGGTCCTGCTCACGCGACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF44 (51710)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475224
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51710 | ZNF44 | zinc finger protein 44 | NM_001164276.2 | 11% | 8.6% | (many diffs) |
| 2 | human | 51710 | ZNF44 | zinc finger protein 44 | XR_002958321.1 | 2.3% | (many diffs) | |
| 3 | human | 51710 | ZNF44 | zinc finger protein 44 | XR_001753701.1 | 2.2% | (many diffs) | |
| 4 | human | 51710 | ZNF44 | zinc finger protein 44 | XR_001753702.1 | 2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 243
- ORF end:
- 483
- ORF length:
- 240
- Sequence:
-
1 TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG 61 GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT 121 TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT 181 TCCATTTCAG GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG 241 GCATGGCCCT GTGTTATGGA ACTTTCTGGG GCTACCCTAA GATGCTGGAA GCTGCCAATC 301 TCATGGAGGG CCTAGTGGAT ATCGGCCCTT GGGTCACTCT TCCCAGAGGA CAGCCTGAGG 361 TGTTGGAGTG GGGCCTCCCA AAGGATCAGG CTGGAGAACT TGGGACAGTG GATAAGTCTG 421 GTCTGTTCCT GTTATCTGGA CTTGGCAGAT TAATGCAACA TTCTGTGGGA CTTTGCAATA 481 GCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 541 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 601 GGCTTTATAT ATCTTGTGGA AAGGACGAGA CGTGGTCCTG CTCACGCGAC GAACGCGTTA 661 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt