Construct: ORF TRCN0000475245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002954.1_s317c1
- Derived from:
- ccsbBroadEn_00325
- DNA Barcode:
- CCCTTATTTTGAAATGTAAGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLIC2 (1193)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475245
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1193 | CLIC2 | chloride intracellular chan... | NM_001289.6 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aggcctgcgg cccggcactc aagtggaccc tgagattgag ctttttgtaa 121 aggctggaag tgatggagag agtattggaa actgtccctt ttgccaacgc cttttcatga 181 tcctctggct taaaggagtt aaatttaatg tgacaactgt tgacatgacc agaaagcctg 241 aagaactaaa ggacttagcc ccaggtacca atcctccgtt cctggtgtat aacaaggagt 301 tgaaaacaga cttcattaaa attgaggagt ttttagaaca aaccctggct cctccaaggt 361 accctcacct gagtcccaag tacaaggagt cttttgatgt gggctgtaac ctctttgcca 421 agttttctgc atacattaag aatacacaaa aggaggcaaa taagaaTTTT GAAAAATCTC 481 TGCTCAAAGA ATTCAAGCGT CTGGATGACT ACTTAAACAC CCCACTTCTG GATGAAATTG 541 ATCCAGACAG TGCTGAGGAA CCCCCAGTTT CCAGAAGACT ATTCTTGGAT GGGGACCAGC 601 TAACACTGGC TGATTGTAGC TTGTTACCCA AGCTGAACAT TATTAAAGTT GCTGCCAAGA 661 AATATCGTGA CTTTGACATT CCAGCAGAAT TCTCAGGAGT CTGGCGTTAT CTCCACAATG 721 CCTATGCCCG TGAAGAATTT ACCCACACGT GTCCTGAAGA CAAAGAAATT GAAAATACTT 781 ACGCAAATGT GGCTAAACAG AAGAGTTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCCTTATT 961 TTGAAATGTA AGGAACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1021 aagatt