Construct: ORF TRCN0000475249
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015684.1_s317c1
- Derived from:
- ccsbBroadEn_08159
- DNA Barcode:
- CAGTCCCCTTTAGGCCATTCGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERO1A (30001)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475249
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30001 | ERO1A | endoplasmic reticulum oxido... | NM_014584.3 | 99.9% | 99.7% | 1366G>A |
| 2 | human | 30001 | ERO1A | endoplasmic reticulum oxido... | XM_011536684.2 | 99% | 98.9% | 673_674insAAGGGACAAGTG;1354G>A |
| 3 | human | 30001 | ERO1A | endoplasmic reticulum oxido... | XM_024449554.1 | 94% | 93.9% | 1210_1296del;1453G>A |
| 4 | mouse | 50527 | Ero1l | ERO1-like (S. cerevisiae) | NM_015774.3 | 86.6% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1470
- ORF length:
- 1404
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ccgcggctgg ggattcttgt ttggcctcct gggcgccgtg tggctgctca 121 gctcgggcca cggagaggag cagcccccgg agacagcggc acagaggtgc ttctgccagg 181 ttagtggtta cttggatgat tgtacctgtg atgttgaaac cattgataga tttaataact 241 acaggctttt cccaagacta caaaaacttc ttgaaagtga ctactttagg tattacaagg 301 taaacctgaa gaggccgtgt cctttctgga atgacatcag ccagtgtgga agaagggact 361 gtgctgtcaa accatgtcaa tctgatgaag ttcctgatgg aattaaatct gcgagctaca 421 agtattctga agaagccaat aatctcattg aagaatgtga acaagctgaa cgacttggag 481 cagtggatga atctctgagt gaggaaacac agaaggctgt tcttcagtgg accaagcatg 541 atgattcttc agataacttc tgtgaagctg atgacattca gtcccctgaa gctgaatatg 601 tagatttgct tcttaatcct gagcgctaca ctggttacaa gggaccagat gcttggaaaa 661 tatggaatgt catctacgaa gaaaactgtt ttaagccaca gacaattaaa agacctttaa 721 atcctttggc ttctggtcaa gggacaagtg aagagaacac tttttacagt tggctagaag 781 gtctctgtgt agaaaaaaga gcattctaca gacttatatc tggcctacat gcaagcatta 841 atgtgcattt gagtgcaaga tatcttttac aagagacctg gttagaaaag aaatggggac 901 acaacattac agaatttcaa cagcgatttg atggaatttt gactgaagga gaaggtccaa 961 gaaggcttaa gaacttgtat tttctctact taatagaact aagggcttta tccaaagtgt 1021 taccattctt cgagcgccca gattttcaac tctttactgg aaataaaatt cagGATGAGG 1081 AAAACAAAAT GTTACTTCTG GAAATACTTC ATGAAATCAA GTCATTTCCT TTGCATTTTG 1141 ATGAGAATTC ATTTTTTGCT GGGGATAAAA AAGAAGCACA CAAACTAAAG GAGGACTTTC 1201 GACTGCATTT TAGAAATATT TCAAGAATTA TGGATTGTGT TGGTTGTTTT AAATGTCGTC 1261 TGTGGGGAAA GCTTCAGACT CAGGGTTTGG GCACTGCTCT GAAGATCTTA TTTTCTGAGA 1321 AATTGATAGC AAATATGCCA GAAAGTGGAC CTAGTTATGA ATTCCATCTA ACCAGACAAG 1381 AAATAGTATC ATTATTCAAC GCATTTGGAA GAATTTCTAC AAGTGTGAAA AAATTAGAAA 1441 ACTTCAGGAA CTTGTTACAG AATATTCATT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1501 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1561 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAGTC 1621 CCCTTTAGGC CATTCGAAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1681 tgaaagatt