Construct: ORF TRCN0000475268
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000495.1_s317c1
- Derived from:
- ccsbBroadEn_13333
- DNA Barcode:
- GCTAACTTTGTGCATGCGTATAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYNE4 (163183)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475268
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 163183 | SYNE4 | spectrin repeat containing ... | NM_001297735.3 | 99.8% | 99.6% | 495G>C |
| 2 | human | 163183 | SYNE4 | spectrin repeat containing ... | NM_001039876.3 | 71.9% | 71.7% | 279_617del;834G>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 939
- ORF length:
- 873
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctgtccctg cctctgggcc ctagacttgg ctcagagccc ctcaaccacc 121 caccgggagc acctagagag gcggacattg ttggatgcac cgtctgcccc gcgtccggag 181 aggagagcac gagcccagag caggcccaga ccctgggaca ggactccttg ggccctcctg 241 agcacttcca gggtgggcca aggggcaatg agcctgccgc tcaccccccg agatggtcaa 301 caccctcttc ctacgaggac ccagctgggg gcaaacactg tgaggtgttc gaggaggcca 361 acacgctgga ccaggacttg gaggtcgagg gagactcgga ctggccagga cctggtgggg 421 tctgggggcc ctgggcaccc agtagcctcc ccacttccac agagttggag tgggatccgg 481 cgggggacat tgggggcctt gggcccttgg gacaaaagac agcccggaca ctaggagtgc 541 cctgtgagct gtgtggccac agggggcccc agggCAGGGG ACAAGGCCTT GAGGAAGCAG 601 ACACCTCTCA CTCCCGACAG GACATGCTGG AGTCTGGCCT CGGCCACCAG AAACGCTTAG 661 CACGTCACCA AAGACACTCC CTGCTCCGGA AGCCTCAGGA CAAGAAGAGG CAAGCATCTC 721 CTCATCTCCA GGATGTGAGG CTGGAGGGGA ATCCAGGGGC CCCCGATCCT GCATCCAGGC 781 AGCCTCTGAC CTTCCTCCTT ATCCTCTTCC TCCTCTTCCT CCTCCTGGTG GGTGCCATGT 841 TTCTCCTGCC CGCGTCAGGA GGCCCCTGCT GCTCTCATGC CCGAATACCC AGGACACCCT 901 ACCTGGTGCT CAGCTATGTC AATGGTCTTC CCCCAGTCTG CCCAACTTTC TTGTACAAAG 961 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1021 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1081 ACGAGCTAAC TTTGTGCATG CGTATAACAC GCGTTAAGTC gacaatcaac ctctggatta 1141 caaaatttgt gaaagatt